Postal development of rats and masometric parameters of internal organs of rats of two generations in the exercise of glifosat-resistants genetically modified soy and roundup
DOI:
https://doi.org/10.31548/bio2018.03.002Abstract
Roundup-resistant genetically modified soybeans are the most widely grown throughout the world, and Roundup is widely used in agriculture. The article presents the results of the study of the effect of traditional, glyphosate-resistant genetically modified soybeans and the effect of the "Roundup" herb on the postnatal development of female rats, as well as on the massometric indices of the internal organs of rats of two generations. Toxic substances can lead to changes in the massometric indices of the internal organs and changes in the postnatal development of rats, therefore the study of the influence of genetically modified soya and herbicide "Roundup" on the body of animals is very relevant.
The research was conducted on the rats of the Vistar line, which were divided into five groups: Ist group - intact group; IInd group - on the diet of rats 20-26% of the feed was replaced by traditional soy; IIIrd group - of the diet, 20-26% of the feed was replaced by genetically modified soy which was not treated with herbicide “Roundup”; IVth group of rats that used genetically modified soy which was treated with a herbicide; Vth group of rats, which received herbicide with drinking water. 42 days after the commencement of traditional and genetically modified soy was used, the females of all groups were mating and continued to receive the same diet and herbicide in drinking water. Postnatal development was studied by the number of litter in each group and analysis of the survival rate of second-generation of rats during the two months, as well as the dynamics of body was weighted after the twenty days and two months of their birth.
Studies have shown that the use of a diet which contained traditional soy and genetically modified, which was not treated with the herbicide “Roundup”, did not lead to changes in postnatal development and changes in massometric indices compared to controls. The analysis of the results of other groups' studies showed that the use of genetically modified soy which was treated with the herbicide “Roundup” and the herbicide with drinking water lead to an increase in the liver mass by 4% and a spleen by 36% in the IVth group of first generation of rats, the same pattern was observed in the second generation of rats. In the Vth group, the weight of the liver increased by 7% and the spleen by 16% compared to the control group of first generation of rats, аnd by 5% and 6% the second generation, and a slightly increased in the mass of the liver and the spleen. In the IVth and Vth group, the fertility rate of the second generation of rats was reduced and their mortality increased during the first two months.
When the herbicide and genetically modified soy was put to the body, and increased in the liver it indicated the toxic effect. The increase of the spleen indicated a negative effect of the roundabout on the immune system.
Key words: massometric indices, postnatal development, traditional and transgenic soy, roundup, genetically modified organisms, coefficients of mass of internal organs.
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