POLYMORPHISM OF ACTIN GENE INTRONS AS AN INSTRUMENT FOR GENOTIVING OF THE REPRESENTATIVES FROM SOLANACEAE FAMILY



DOI: http://dx.doi.org/10.31548/biologiya2018.287.071

А. S. Postovoitova, Ya. V. Рirko, Ya. B. Blume

Анотація


In modern selection, DNA markers are an indispensable tool for identifying and selecting genotypes of economically valuable crops. New and promising direction of development of molecular markers is a gene-targeted markers (GTMs), namely markers, which are based on the identified intron length polymorphism of genes (ILP). The aim of the study is to evaluate the usefulness of the length polymorphism of the second actin gene intron (АВР-actinbasepolymorphism) for DNA-profiling  varieties of plants from family Solanaceae.DNA was isolated from seedlings using CTAB-method. PCR was conducted using our own universal АВР primers. The fragments were separated by electrophoresis in a 6% polyacrylamide gel, and visualized by silverstains. 12 varieties of tomato (S. lycopersicum) and 4 potato varieties (S. tuberosum) were analyzed. Each tomato variety contained at least 7 fragments of II-nd intron, but only one polymorphic bend was detected. The АВР-profiles of the potato varieties were more heterogeneous. The different varieties of tomatoes and potatoes were identificated by ABP marker system. It was shown the significant difference between the profiles of the analyzed species. In general, ABP-markers are a reliable source of genetic information and can be widely used for genotyping and evaluating of the differentiation of commercially valuable plantsof Solanaceae.


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