MODIFICATION OF TRANSDUSER SURFACE OF BIOSENSORS ON THE BASIS OF PPR FOR DIAGNOSTICS OF LEUCOSE CATTLE



DOI: http://dx.doi.org/10.31548/biologiya2018.287.100

M. F. Starodub, M. I. Fedesh-Gladinets, M. V. Savchuk, O. P. Taran

Анотація


Leucosis of BLV is a widespread chronic disease of the tumor nature, occurs asymptomatically or is characterized by lymphocytosis and growth of lymphoid cells in various organs of animals. Such a disease brings huge losses in agricultural holdings. The main measure for overcoming this disease is timely diagnosis and treatment. Common methods of diagnosing leukemia are the methods of RID and ELISA. However, these methods are long-term and require research in the laboratory. Therefore, at present, it is urgent to develop new instruments for rapid diagnosis of the condition of animals in the field. The aim of the work was to work out the main approaches to the functionalization of transducer surfaces to increase the biosensor signal for the diagnosis leucosis of BLV . Immunological and statistical methods of research were used in the work. By comparing the signals of the biosensor based on PPR after its functionalization with recombinant antigens gp51 and p24, it was established that the creation of an intermediate layer on the surface of the transducer in general, and especially when using PAA (level with dodecanthiol) allows not only to increase the response, but also to achieve its necessary reproducibility on for at least 4 months. Derivatives of dextran also increase the recall of the biosensor, but do not ensure its stability during storage of the functionalized surface.


Повний текст:

PDF

Посилання


Gulyukin, M. I., Zamaraeva, N. V., Abramov, V. N., Barabanov, N. I. (1999). Sostoyanie i perspektiva borbyi s leykozom krupnogo rogatogo skota [The state and prospects of combating leukemia of cattle]. Veterinariya, 12, 3-8.

Mandigra, M. (2000). GenetichnI aspekti leykozu velikoyi rogatoyi hudobi [Genetic aspects of leukemia in cattle]. Vet. med. UkraYini, 18-19.

Yarchuk, B. M., Dombrovskiy, O. B., TirsIn, R. V., Kornienko, L. E., Dovgal, O. V. (2000). Leykoz velikoyi rogatoyi hudobi [Leukemia of cattle]. Kyiv: Biblioteka vet. med., 9-12, 62 s.

Pirogova, L. V, Starodub, M. F. Artyuh, V. P., Nagaeva, L. I., Dobrosol, G. I. (2002). Ekspresna diagnostika leykozu velikoyi rogatoyi hudobi za dopomogoyu imunnogo sensora na osnovi poverhnevogo plazmonnogo rezonansu [Extreme diagnosis of leukemia in cattle using an immune sensor based on surface plasmon resonance]. Ukr. bшohшm. Zhurn, 74, 3, 88 – 92.

Starodub, N. F., Pirogova, L. V. (2003). Instrumentalnaya ekspress-diagnostika leykozov na osnove biosensornoy tehnologii[Instrumental express diagnostics of leukemia based on biosensor technology]. Sbornik: «Bioresursyi, biotehnologii, innovatsii yuga Rossii», Materialyi Mezhdunarodnoy nauchno-prakticheskoy konferentsii 21-24 oktyabrya 2003, Stavropol Pyatigorsk, 2, 144-149.

Starodub, M. F., Pirogova, L. V. (2007). Sposib neinvaziynoyi diagnostiki leykozu velikoyi rogatoyi hudobi shlyahom viznachennya antitil do virusu u molotsi koriv imunosensorom poverhnevogo plazmonnogo rezonansu [Method of non-invasive diagnosis of bovine leukemia by detection of antibodies to a virus in milk of cows with an immunosensor of surface plasmon resonance]. Patent Ukrayini na vinahid, №12689/3730, Byul. vId 23.11.2007.

Starodub, N. F., Starodub, V. N. (2003). Infektsionnyiy leykoz krupnogo rogatogo skota i ego diagnostika[Infectious leukemia of cattle and its diagnosis]. Biopolimeryi i kletka, 19,4, s. 307-316. https://doi.org/10.7124/bc.000663


Метрики статей

Завантаження метрик ...

Metrics powered by PLOS ALM

Посилання

  • Поки немає зовнішніх посилань.