USE OF ISSR ANALYSIS FOR STUDYING THE INTRANAL-GENETIC POLYMORPHISM OF DIFFERENT GROUPS OF CULTURAL POPULATION OF WILS OF THE GENUS EISENIA WORMS



DOI: http://dx.doi.org/10.31548/biologiya2018.287.151

K. I. Timchy, E. I. Metlitskaya, S. M. Korennay, V. T. Smetanin

Анотація


It is known that molecular genetic methods can provide researchers with new and more accurate means for distinguishing between types of earthworms and establishing phylogenetic distances between them.

Using neutral molecular markers, ISSR (Inter-SimpleSequenceRepeat), internal genetic genetic polymorphism of earthworms of the genus Eisenia was studied from the mass of the vermiculture of the biotechnology department, which were irradiated with a laser of the LGH-208b type at different exposures over time and cultivated on various organic substrates.

The application of the ISSR-PCR method is the use of microsatellite loci as areas for firing primers that are complementary to microsatellite repeats. Such primers allow the amplification of DNA fragments that are located between the microsatellite sequences. The resulting patterns are species specific.

Studies have shown that the animal line has a fairly high degree of heterogeneity, despite the fact that it occurs from six individuals of the same massif, it is the effect of laser irradiation and the hard growing substrate (bottom deposits) that have proved to be powerful selective pressure factors.


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