Seed transmission of bean common mosaic virus in phaseolus vulgaris cv Chervona Shapochka



DOI: http://dx.doi.org/10.31548/biologiya2020.01.069

A. Kyrychenko, K. Hrynchuk, I. Antipov, A. Likhanov

Анотація


Abstract. Bean common mosaic virus (BCMV) is one of the most harmful and widespread bean viruses. The pathogen can be transmitted with seeds and pollen with a fairly high frequency. With efficient spread by vectors to susceptible crops, even a low level of seed infection can lead to an epidemic situation. In Ukraine BCMV is widespread in all dry bean growing areas and may cause serious crop losses. The aim of this study was to investigate the level of Bean common mosaic virus seed transmission in Phaseolus vulgaris cv Chervona shapochka. Whereas high incidence of seed transmission occurred in direct relation to virus invasion of an immature embryo we tended to follow the virus infection in reproductive tissues of bean. To determine the possibility of vertical transmission of BCMV via pollen, detection of virus RNA in pollen grains by PCR has been carried out. In the study biological methods (mechanical inoculation of viruses, detection of virus infectivity in indicator plants) and molecular biological techniques (total RNA extraction from the plant tissues, reverse transcription polymerase chain reaction) were used. It was shown P. vulgaris cv Chervona shapochka transmitted the BCMV strain in 77 % of the seed produced by infected plants. The date obtained indicates a high level of seed transmission of the virus. According to the findings virus infected seeds have a great value in disease spreading. The viral RNA has been detected in plant generative organs and some components of the seed and flower. Also virus particles were found in pollen grains of Phaseolus vulgaris plants. Diffuse granular viral inclusions were detected in the cytoplasm and nucleus of developing embryo cells.


Ключові слова


Keywords: Bean common mosaic virus (BCMV), seed infection, reverse transcription polymerase chain reaction (RT-PCR)

Повний текст:

PDF (English)

Посилання


Antipov, I., Hrynchuk, K., Duplyak, О. (2016). Rozrobka PLR-system dlya identyfikatsiyi virusu zvychaynoyi mozayiky kvasoli [Development of PCR systems for identification of bean common mosaic virus]. Naukovyy visnyk NUBIP Ukrayiny - Scientific herald of NULES of Ukraine. Series: biology, biotechnology, ecology. 234, 40-46 [in Ukrainian]. Режим доступу: http://nbuv.gov.ua/UJRN/nvnau_biol_2016_234_7.

Bos, L. (1971). Bean Common Mosaic Virus. C.M.I. A.A.B. Description of Plant Viruses, 73, 4. режим доступу: http://www.dpvweb.net/dpv/showdpv.php?dpvno=073

Deligoz, I., Soken, M.A. (2013). Differentiation of Bean Common Mosaic Virus (BCMV) and Bean Common Mosaic Necrosis Virus (BCMNV) strains infecting common bean in Samsun province. J Turk Phytopath. 7, 1-14. режим доступу: https://dergipark.org.tr/en/download/article-file/72887

Dospekhov, B.A. (1985). Metodika polevogo opyta (s osnovami statisticheskoy obrabotki rezul'tatov issledovaniy) [Field Experience Method]. Agropromizdat, Moscow. 416 p. [in Russian].

Goldin, M.I. (1963), Virusnye vkljuienija v rastitelnoj kletke i priroda virusov. [Viral inclusions in plant cell and the nature of viruses]. Izdat. AN SSSR, Moskva - Akad. Nauk USSR, Moscow. 204 p. [in Russian].

Hampton, R., Waterworth, H., Goodman, R.M., Lee, R. (1982). Importance of seed-borne viruses in crop germplasm. Plant Dis. 66, 977-978. режим доступу: http://europepmc.org/abstract/AGR/IND82118564

https://doi.org/10.1094/PD-66-977

Hema, M., Sreenivasulu, P., Patil, B.L., Kumar, P.L., Reddy, D.V.R. (2014). Tropical food legumes: virus diseases of economic importance and their control. Adv Virus Res. 90, 431-505.

https://doi.org/10.1016/B978-0-12-801246-8.00009-3

Kyrychenko, A.N., Kovalenko, A.G. (2018). Bean common mosaic in the Kiev region: etiology of disease and pathogen identification. Mikrobiol. Z.; 80(4), 96-107.

https://doi.org/10.15407/microbiolj80.04.096

Maule, A.J., Wang, W. (1996). Seed transmission of plant viruses: a lesson in biological complexity. Trends in Microbiol. 4(4), 153-158. https://doi.org/10.1016/0966-842X(96)10016-0

Mockovets', S.M., Kraev, V.G., Porembc'ka, N.B., Bilyk, L., Baratova, D.F. (1971). Virusy ta virusni zakhvoryuvannya kulʹtur bobovykh v Ukrayini [Viruses and virus diseases of legume cultures in Ukraine]. Naukova dumka, Kyiv. 136 p. [in Ukrainian].

Nordenstedt, N., Marcenaro, D., Chilagane, D., Mwaipopo, B., Rajamaki, M.L., Nchimbi-Msolla, S., Njau, P.J.R., Mbanzibwa, D.R., Valkonen, J.P.T. (2017). Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania. PLoS ONE. 12:e0178242. https://doi.org/10.1371/journal.pone.0178242

Pausheva Z.P. Praktikum po tsitologii rasteniy. - 4-e izd., pererab. i dop. - M.: Agropromizdat, 1988. - 271 s.;

Sastry, K.S. (2013). Seed-borne plant virus diseases. Springer Publishers, New Delhi, pp 327.

https://doi.org/10.1007/978-81-322-0813-6

Schmidt, H.E. (1992). Bean mosaics. In: Chaube H.S., Singh U.S., Mukhopadhyay A.N., Kumar J., editors. Plant Diseases of International Importance. Vol. II, Prentice Hall, New Jersey; 40-73.

Sipahioğlu, H.M., Ocak M., Usta M. (2007). Comparison of three conventional extraction methods for the detection of plant virus/viroid RNAs from heat dried high-phenolic host leaves. Asian J. Plant Sci. 6(1), 102-107.

https://doi.org/10.3923/ajps.2007.102.107

State register of plant varieties suitable for dissemination in Ukraine in 2018. (2018). Retrieved from https://sops.gov.ua/uploads/page/5bbdf6a297647.pdf [in Ukrainian]

Varma, A. (1988). The economic impact of filamentous viruses. In: Milne R.G. editor. The Plant Viruses: The Filamentous Plant Viruses, Plenum Press, New York and London; p. 331-407.

https://doi.org/10.1007/978-1-4684-7038-3_11

Walters, E.C. (1962a). Thirty eight years behind the times and still they germinate. Assoc Off Seed Anal News. 36, 8.

Worrall, E.A., Wamonje, F.O., Mukeshimana, G., Harvey, J.J.W., Carr, J.P., Mitter N. (2015). Bean common mosaic virus and Bean common mosaic necrosis virus: relationships, biology and prospects for control. Adv. Virus Res. 93, 1-46.

https://doi.org/10.1016/bs.aivir.2015.04.002


Метрики статей

Завантаження метрик ...

Metrics powered by PLOS ALM

Посилання

  • Поки немає зовнішніх посилань.