MAIN ASPECTS TRANSFER FOREIGN GENES IN THE GENOME OF SPERM JAPANESE QUAIL (COTURNIX JAPONICA)



Y.І. Lesniak, L.I. Kalakaylo, V.G. Spiridonov, M.D. Melnichuk

Анотація


This paper describes a process of transferring genetic construction (pEGFP-N1 vector) into the genome of Japanese quail sperm using dimethyl sulfoxide (DMSO). Transfer and expression of the introduced genetic information was evaluated by the characteristic fluorescence of transfected spermatozoa due to expression of the green fluorescent protein (GFP) and by PCR analysis.


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