CYTOLOGICAL CHANGES IN ELODEA CANADENSIS DURING BIOTESTING OF CHRONIC TOXICITY OF SURFACE WATER MONITORED IN PRYPIAT RIVER RIGHT-BANK TRIBUTARIES
Анотація
The aim of the presented work was to examine the cytological changes in Elodea canadensis after exposure of different duration during the chronic toxicity assessments of river surface water for different hydrological seasons.
The experiments were carried out at different exposure times (7, 14, 21 days). In each series of experiments, the total number of cytological changes of E. canadensis was higher in summer compared to winter. The results of toxicity biotesting of surface water in representative control sites in most cases were statistically significant compared with control (p≤0.05) in case of the 14-day exposure.
The researcher conducted a correlation analysis between the water quality indicators and biotesting parameters. Nonetheless, the total number of cytological changes in the test object was characterized by the most numerous dependencies with the medium strength of relationship. Those pairs of variables included the following: suspended solids (r=0.67), ammonia nitrogen (r=0.56), nitrate-nitrogen (r=0.59), the BOD5 rate (r=0.67), the content of dissolved oxygen (r=-0.60), zinc (r=0.52), and manganese (r=0.61).
Basing on the obtained results, the author suggests using cytological changes coefficient for the E. canadensis test object as a summarizing indicator within the biological assessment of surface water chronic toxicity. The noted indicator presents the ratio of the number of cells that are in a state of necrosis, initial necrosis and isotonic plasmolysis to the total number of analysed cells, while taking into account the duration of the plants’ exposure to the studied water samples.
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