STEVIA POLYPLOIDIZATION IN VITRO
DOI:
https://doi.org/10.31548/dopovidi2016.06.013Keywords:
polyploidization, colchicines, stevia, in vitro, tetraploidsAbstract
On the basis of in vitro studies the methodology of creation of tetraploids stevia. For their creation in vitro culture used diploid selection number of stevia 1216, 15-15/3, 112, 116, which are resistant to diseases and have high stevioside content; the cultivation was conducted under the conditions of temperature 24±2°C, lighting 3000-4000 Lux, photoperiod ‒ 16 hours on a shaker in 100 mode.min For clonal micropropagation of shoots, treated with 0.02% colchicine, separately transplanted in the medium of agar-agar. After 5 days’ cultivation, newly formed shoots are separated, are registered with the definition of vegetative generations Kohler escape. The second generation of vegetative shoots, in the amount of not less than 3 Grand, after two weeks of culturing were used for the determination of ploidy and selection of tetraploids. To synchronize cell division of meristem flasks were placed in a refrigerator for 12 hours at a temperature of +4 °C, and then 2.5-3 hours on light to obtain 3000-4000 Lux. In such conditions, get up to 37% of ploidy tetraploid forms of stevia with high productivity and resistant to fungal diseases.
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