KARIOTYP ANALYSIS OF MESENCHIMAL BONE MARROW STEM CELLS OF RABBITS BY DIFFERENT METHODS DISSOCIATION OF CELL MONOLAYERS AT EARLY CULTIVATION PASSAGE IN VITRO



А. Y. Mazurkevych, М. О. Maliuk, L. F. Starodub, V. B. Danilov

Анотація


Conducted by us researches showed that in the culture of stem cells, obtained by the method of enzymatic dissociation of cell material much larger number of cells with chromosomal disorders apparatus quantitative nature (aneuploidy, polyploidy), compared to the culture stem cells obtained by the method dissociation of chelating cellular material. Especially clearly such quantitative disorders are manifested in cell culture III and V of passages. According to the analysis micronucleus test we installed, that in culture of stem cells obtained by the method of enzymatic dissociation, the number of cells with mutated significantly exceeded the level of spontaneous


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