The influence of Pseudomonas Fluorescens Bacteria and substances of stimulating nature on the productivity of the soybean plant and the damage of seeds by pathogens
DOI:
https://doi.org/10.31548/agr2021.04.028Keywords:
bacteria, plant productivity, seed infestation, fungiAbstract
Soybeans are a strategic legume for global agriculture and play an important role in ensuring the food security of many countries. At the same time, biotic factors, in particular pathogens, prevent high and high-quality crop yields. Their control is carried out using various methods and techniques. However, with an excessive increase in the use of chemical pesticides, the ecological situation in agrocenoses becomes more complicated. Therefore, it is important to reduce the pesticide load in crop production technologies.
The aim of the research is to evaluate the influence of Pseudomonas fluorescens bacteria and substances of stimulating nature on the productivity of soybean plants and seed damage by pathogens.
The experiments were performed in the conditions of the Ukrainian Plant Quarantine Research Station of the Institute of Plant Protection of NAAS. The soil of the experimental plot is gray forest podzolic heavy loam. Xenia soybean variety was used for research. Field research was performed according to generally accepted methods. Soybean crops were sprayed during the growing season in the following periods: true trifoliate leaf, flowering and bean formation. For the treatment of soybean plants used a preparation based on bacteria Pseudomonas fluorescens with a titer of 3 x 109 CFU/cm3 with a rate of 5.0 l/ha when used in combination with substances with stimulant action. Over the years of research, the amount of precipitation (in some months of the growing season of soybean plants) was higher than the average monthly long-term norm. Pre-harvest desiccation of crops was not performed. Phytopathological examination of seeds obtained from soybean plants under different treatments was carried out according to DSTU 4138-2002.
Studies have shown a positive effect of the drug based on P. fluorescens and substances of a stimulating nature on the biometric parameters of soybean plants and their productivity. In particular, the weight of 1000 seeds in the variants with spraying of plants increased in the range from 16 to 66 g. There was also a decrease in infection of soybean seeds with pathogens of fusarium wilt, Alternaria and penicillosis - fungi Fusarium oxysporum Schlecht, Alternaria spp. and Penicillium expansum Link. The study of immunoprotective and stimulating effects of combinations of biocomplexes is promising for the development of environmentally friendly measures to increase soybean yield and reduce seed damage by pathogens.References
Michelfelder A. J. (2009). Soy: A complete source of protein. American Family Physician. Vol. 79, № 1. P. 43-47.
https://doi.org/10.1016/S1877-573X(09)00055-0
Cherenkov A.V. et al. (2014). Zernobobovi kulʹtury: suchasni tekhnolohiyi vyroshchuvannya [Legumes: modern technologies of cultivation]: monohrafiya. Dnipropetrovsk: Akcent. 109 p.
Babych A. A, Babych-Poberezhna A. A. (2012). Svitovi ta vitchyznyani tendentsiyi rozmishchennya vyrobnytstva i vykorystannya soyi dlya rozvʺyazannya problemy bilka [World and domestic trends in the location of production and use of soybeans to solve the problem of protein]. Kormy i kormovyrobnytstvo. V. 71. S. 12-25.
De Maria M., Robinson E. J. Z., Kangile J. R., Kadigi R., Dreoni I., Couto M., Howai N., Peci J., Fiennes S. (2020). Global Soybean Trade. The Geopolitics of a Bean. UK Research and Innovation Global Challenges Research Fund (UKRI GCRF) Trade, Development and the Environment Hub. https://doi.org/10.34892/7yn1-k494
Berbenets O. V. (2019). Svitove vyrobnytstvo soyi yak nevycherpnoho dzherela bilkiv roslynnoho pokhodzhennya ta mistse Ukrayiny na svitovomu rynku torhivli neyu [World soybean production as an inexhaustible source of proteins of plant origin and Ukraine 's place in the world market of its trade]. Ahrosvit. № 10. S. 41-45.
https://doi.org/10.32702/2306-6792.2019.10.41
Pikovskyi M. Y., Kyryk M. M. (2012). Symptomatyka biloyi hnyli soyi [Symptoms of white mold of soybeans]. Karantyn i zakhyst roslyn. № 7. S. 2-5.
Baetsen-Young A. M., Swinton S. M., Chilvers M. I. (2021). Economic Impact of Fluopyram-Amended Seed Treatments to Reduce Soybean Yield Loss Associated with Sudden Death Syndrome. Plant Dis. 105(1). P. 78-86.
https://doi.org/10.1094/PDIS-04-20-0792-RE
Díaz-Cruz G. A., Cassone B. J. (2021). Amplicon Sequencing Reveals Extensive Coinfections of Foliar Pathogens in Soybean. Plant Disese. Vol.105, № 1. P. 127-133.
https://doi.org/10.1094/PDIS-06-20-1165-RE
Dorrance A. E., Robertson A. E., Cianzo S., Giesler L. J., Grau C. R., Draper M. A., Tenuta A. U., Anderson T. R. (2009). Integrated management strategies for Phytophthora sojae combining host resistance and seed treatments. Plant Disease. Vol. 93. P. 875-882.
https://doi.org/10.1094/PDIS-93-9-0875
Kurle James, Grau Craig, Oplinger Edward, Mengistu Alemu. (2001). Tillage, Crop Sequence, and Cultivar Effects on Sclerotinia Stem Rot Incidence and Yield in Soybean. Agronomy Journal. Vol. 93, № 5. P. 973-982.
https://doi.org/10.2134/agronj2001.935973x
Reznikov S., De Lisi V., Claps P., González V., Devani M. R., Castagnaro A. P., et al. (2019). Evaluation of the Efficacy and Application Timing of Different Fungicides for Management of Soybean Foliar Diseases in Northwestern Argentina. Crop Protection. Vol. 124.
https://doi.org/10.1016/j.cropro.2019.104844
Baranov V. F., Makhonin V. L. (2014). O biologicheskoy zashchite agrofitotsenozov soi ot vrednykh organizmov [About biological protection of soybean agrophytocenoses from harmful organisms]. Maslichnyye kul'tury. № 1. S. 152-164.
Chandrashekara K. N., Manivannan S., Chandrashekara C., Chakravarthi M. (2012). Chapter: 10. Biological Control of Plant Disease. Eco-friendly Innovative Approaches in Plant Disease Management. Editors: Vaibhav K. Singh, Yogendra Singh, Akhilesh Singh. Publisher: International Book Distributors. Р.147-166.
Alani Rakib, Adhab Mustafa, Mahdi Haider, Abood Hadi. (2012). Rhizobium japonicum as a Biocontrol Agent of Soybean Root Rot Disease Caused by Fusarium solani and Macrophomina phaseolina. Plant Protection Science. Vol. 48, № 4. P. 149-155.
https://doi.org/10.17221/16/2012-PPS
Arfaoui A., Adam L.R., Bezzahou A. et al. (2018). Isolation and identification of cultivated bacteria associated with soybeans and their biocontrol activity against Phytophthora sojae. BioControl. Vol. 63. P. 607-617.
https://doi.org/10.1007/s10526-018-9873-9
Susilowati Ari, Wahyudi Aris, Lestari Yulin, Suwanto Antonius, Wiyono Suryo. (2013). Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi. HAYATI Journal of Biosciences. Vol.18, № 2. Р. 51-56.
https://doi.org/10.4308/hjb.18.2.51
Zhou Keqin, Yamagishi Masumi, Osaki Mitsuru. (2008). Paenibacillus BRF‐1 has biocontrol ability against Phialophora gregata disease and promotes soybean growth. Soil Science & Plant Nutrition. № 54. Р. 870-875.
https://doi.org/10.1111/j.1747-0765.2008.00308.x
Jahan Akida, Jahan Nushrat, Hossain Mohammad, Hossain Muhammed. (2015). Biological control of anthracnose of soybean. Research in Agriculture Livestock and Fisheries. Vol. 2, № 3. P. 419-426.
https://doi.org/10.3329/ralf.v2i3.26164
Zhang Fuli, Ge Honglian, Zhang Fan, Guo Ning, Wang Yucheng, Chen Long, Ji Xiue, Li Cheng-Wei. (2016). Biocontrol Potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean. Plant Physiology and Biochemistry. Vol. 100. P. 64-74.
https://doi.org/10.1016/j.plaphy.2015.12.017
Solomiychuk M. P., Kordulyan Y. V., Melnyk A. T., Pikovskyi M. Y. (2020). Vplyv biolohichnykh kompleksiv ta biostymulyuyuchykh rechovynna rist i rozvytok roslyn soyi v Zakhidnomu Lisostepu Ukrayiny [Influence of biological complexes and biostimulating material growth and development of soybean plants in the Western Forest-Steppe of Ukraine]. Peredhirne ta hirsʹke zemlerobstvo i tvarynnytstvo. V. 67 (2). S. 182-197.
Kabanets V. M., Sobko M. H., Murach O. M. (2017). Funktsionuvannya symbiozu «Japonicum - soya» i vrozhaynistʹ soyi za vplyvu ryzohuminu ta fiziolohichno aktyvnykh rechovyn [Functioning of symbiosis "Japonicum - soy" and soybean yield under the influence of rhizohumin and physiologically active substances]. Kormy i kormovyrobnytstvo. V. 83. S. 58-66.
Tribel S. O, Sigareva D. D., Sekun M.P., Ivashchenko O.O. et al. (2001). Metodyka vyprobuvannya i zastosuvannya pestytsydiv [Methods of testing and application of pesticides]. Kyiv: Svit. 448 s.
Nasinnya silʹsʹkohospodarsʹkykh kulʹtur. Metody vyznachennya yakosti: DSTU 4138-2002 [Seeds of agricultural plants. Methods for seed testing]. Kyiv: Derzhspozhyvstandart Ukrayiny, 2003. 173 s.
Kyryk M. M., Pikovskyi M. Y., Azaiki S. Khvoroby nasinnya silʹsʹkohospodarsʹkykh kulʹtur [Diseases of seeds of agricultural crops]. Kyiv: CP Komprynt. 2015. 340.
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