IMMUNE BIOSENSORS FOR DIAGNOSTICS AND CONTROL OF SALMONELLA TYPHIMURIUM

Authors

  • Yuliia Ogorodniichuk National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • Nikolay Starodub National University of Life and Environmental Sciences of Ukraine image/svg+xml

Abstract

Several types of biosensors based on optical (SPR, TIRE), potentiometric (ISFET) transducers and one based on ZnOnanorods are proposed for S. typhimurium detection. An effect of preliminary preparation of transducers to the sensitivity of analysis was analyzed. It was found that all proposed biosensors were effective for S. typhimurium detection in model solutions and can be used in practice at different levels of diagnostics. The highest sensitivity was obtained using TIRE based biosensor that was several cells (less than 5) in 10 ml.

Author Biographies

  • Yuliia Ogorodniichuk, National University of Life and Environmental Sciences of Ukraine
    PhD student
  • Nikolay Starodub, National University of Life and Environmental Sciences of Ukraine

    doctor of biological sciences, professor

     

References

Zaritskiy A. Salmonellosis / А. М. Zaritskiy – K.: Zdorov'ya, 1988. – 160 p.

Mandate of the Ministry of Health of Ukraine №400 12.05.2010. On approval of the State sanitary rules and regulations “Hygienic requirements to drinking water intended for human consumption” [Electronic source]: / Verkhovna Rada of Ukraine1994-2015. – Access mode: http://zakon4.rada.gov.ua/laws/show/z0452-10/page.

Optical immune biosensor «Plasmonotest» for Salmonella typhimuriumdetection / I. Ogorodniichuk, Т.Lebedeva, P. Shpylovyy, М.Starodub // Sensor Electronics and Мicrosystem Technologies. – 2013. – T. 10 (1), P 106-113.

Application of Room Temperature Photoluminescence from ZnONanorodsfor for Salmonella Detection / Viter R, Khranovskyy V, Starodub N. [et al.] // IEEE Sensors Journal. – V. 14 (6). – 2014. – P. 2028 – 2034.

Barakat S. M. Mahmoud. Salmonella – A Dangerous Foodborne Pathogen. / Barakat S. M. Mahmoud. – InTech. 2012. – 238 р.

Development of indirect competitive ELISA for the detection of Salmonella typhimurium / Bang J., Shukla S., Kim Y., Kim M. // Romanian Biotechnological Letters. – 2012. – V.17 (2). – P 7194-7204.

Ivnitski D. Biosensors for detection of pathogenic bacteria / D. Ivnitski, I. Abdel-Hamid, P. Atanasov, E. Wilkins // J. Biosensors & Bioelectronics. – 1999. – Vol. 14. – P. 599–624.

Nanoparticles formation by combustion techniques: Gaseous dispersed synthesis of refractory oxides / A. N. Zolotko, N. I. Poletaev, J. I. Vovchuk, A. V. Florko // Gas Phase Nanoparticle Synthesis. – 2005. – P. 123–156.

PCR-Genosensor Rapid Test for Detecting Salmonella / Pividori I, Merkoci A, Barbe J, Alegret S. // J Electroanalysis. – 2003. – №15. – P. 23-24.

Prusak-Sochaczewski E. An improved ELISA method for the detection of Salmonella typhimurium / Prusak-Sochaczewski E., Luong J.H.T. // J. Appl. Microbiology. – 1989. – V. 66 (I 2). – P. 127–135.

Downloads