Antagonistic activity of dominant strains of bacteria of spring barley rhizosphere against phytopathogenic micromycetes

Authors

  • M. O. Kiroiants National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • M. V. Patyka National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • T. I. Patyka National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/agr2021.02.054

Keywords:

Bacillus methylotrophicus 10, Phyllobacterium ifriqiyense 1, antagonistic properties, phytopathogenic micromycetes

Abstract

The purpose of the work. The aim of the study was to conduct research on the manifestation of the dominant representatives of the bacterial biome of the rhizosphere of spring barley antagonistic activity against phytopathogenic micromycetes.

Research methods. The standard diffusion method of double culture in Petri dishes was used to study the antagonistic properties of the dominant strains of rhizosphere bacteria. The level of antagonistic activity of microorganisms was assessed by the indicator (%) of inhibition of growth and development of the mycelium of micromycetes Fusarium sporotrichioides Sherb. 23.2, Alternaria alternata (Fr.) Keissl. 3.45, Nigrospora oryzae (Berk. & Broome) Petch. 18.77.

Results and scope of their application. Studies have shown that Bacillus methylotrophicus 10 had an inhibitory effect on Fusarium sporotrichioides Sherb. 23.2 ‒ 77,4%, Alternaria alternata (Fr.) Keissl. 3.45 – 66,6% and Nigrospora oryzae (Berk. & Broome) Petch. 18.77 – 86,7%, while the bacteria Phyllobacterium ifriqiyense 1 showed activity against phytopathogens by 45,1%, 63,1% and 65,0%, respectively.

Conclusions. Thus, both strains of rhizosphere dominant bacteria have high antagonistic activity against phytopathogenic micromycetes, which gives grounds for their further study.

References

Andreiuk, K. I., & Valahurova, E. V. (1992). Fundamentals of the ecology of soil microorganisms. Naukova dumka.

Artamonova, M. N., Alekseeva, A. S., & Potaturkyna-Nesterova, N. I. (2013). Antagonistic activity of associative rhizobacteria. International Journal Of Experimental Education, 10, 276-279.

Dobrovolskaia, T. G. (2002). The structure of soil bacterial communities. M.: ICC Akademkniga.

Ehorov, N. S. (2004). Antibiotic Fundamentals. M.: Publishing house of Moscow State University.

Hadzalo, Y. M., Patyka, M. V., & Zvaryshniak, A. S. (2015). Agrobiology of the rhizosphere of plants. Agricultural science.

Kiroiants, M. O., Patyka M. V., & Patyka, T. I. (2020). Phylogenetic analysis of dominant microorganisms of the genera Bacillus and Phyllobacterium isolated from the rhizosphere of spring barley. Bulletin of Agricultural Science. 5, 48-53. https://doi.org/10.31073/agrovisnyk202005-06

Kriuchkova, L. O., Drahovoz, I. V., Lapa, S. V., & Avdieieva, L. V. (2012). Antagonistic activity of bacteria of the genus Bacillus against phytopathogenic micromycetes. Achievements and problems of genetics, selection and biotechnology. Kyiv. Logos. 4. 368-371.

Novykova, I. I., & Lytvynenko, A. I. (2011). Biological effectiveness of biological products based on antagonistic microbes against root rot of cucumber and strawberry wilt and their effect on the species composition of soil micromycetes. Plant Protection Bulletin. 2. 10-22.

Aeron, A., Pandey, P., & Kumar, S. (2011). Emerging role of plant growth promoting rhizobacteria. Bacteria in Agrobiology: Cropecosystem. Maheshwari, Berlin, Heidelberg: Springer Verlag, D.K.1-26. https://doi.org/10.1007/978-3-642-18357-7_1

Berg, G., Rybakova, D., Grube, M., & Koberl, M. (2016). The plant microbiome explored: implications for experimental botany. J. Exp. Bot. 4. 995-1002. https://doi.org/10.1093/jxb/erv466

Goudjal, Y. (2016). Potential of endophytic Streptomyces spp. for biocontrol of Fusarium root rot disease and growth promotion of tomato seedlings. Biocontrol Sci Technol. 12. 1691-1705.

https://doi.org/10.1080/09583157.2016.1234584

Patyka, N.V., Tonkha, O.L., & Sinchenko, V.N. (2019). Features of the formation of the structural and functional composition of the microbiome of chernozem virgin in the steppe of Ukraine. Mikrobiolohichnyi Zhurnal. 4. 90-106.

https://doi.org/10.15407/microbiolj81.04.090

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Published

2021-08-31

Issue

Vol. 12 No. 2 (2021)

Section

Ґрунтознавство та агрохімія

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