Influence of vicasol on the krebs cycle dehydrogenases activity and state of antioxidant defense system of the smooth muscle tissue of geese
DOI:
https://doi.org/10.31548/bio2019.05.002Keywords:
vicasol, dehydrogenases, antioxidant enzymes, product of lipids peroxidationAbstract
The aim of work was to study the effect of vicasol on the Krebs cycle dehydrogenase activity and the state of the antioxidant defense system of geese muscle tissue of the stomach.
According to the results of the work, it was found in the smooth muscle tissue of the stomach of geese, vicasol increases the activity of glutathione peroxidase, catalase and superoxide dismutase on the 14th and 21st days of ontogenesis, in particular, on the 14th day glutathione peroxidase, catalase and superoxide dismutase activity increase by 181.1 % (p≤0.05), 153.2% (p≤0.05) i 103.3% (p≤0.05), after 7 days 168.0% (p≤0.05), 99.7% (p≤0.05) i 111.3% (p≤0.05) relative to the control group. The antioxidant system enzymes activity of the experimental group on the 28th day tends to decrease, however, a significant difference is observed only between groups of animals for superoxide dismutase, whose activity is reduced by 62.5 % under the influence of vicasol (p≤0.05). On the 35th day of ontogenesis, the activity of glutathione peroxidase and catalase in the experimental group increases by 43.2% (p≤0.05) and 54.1% (p≤0.05) compared to the control, at this time the activity of superoxide dismutase is lower by 23.9 % (p≤0.05).
A significant increase in the concentration of lipids hydrogen peroxides (28th day) by 27% (p≤0.05) relative to the control was established in the tissue, followed by a decrease on the 35th day of ontogenesis. Vicasol stabilizes the content of end products of lipid peroxidation in tissue homogenate, with the exception of a decrease of 8.6% (p≤0.05) at the end of experiment. Upon induction of peroxide processes with Fe2+ in cell, it is increased their content by 108 % (p≤0.05) (21st day), at the end of the experiment, a decrease in the content of secondary lipid decomposition products in the experimental group of animals by 27.5% (p≤0.05).
The antioxidant activity of the tissue at the beginning of the experiment under the influence of vicasol was higher by 30%, on the the 21st day decreased by 50% relative to the control group, and on the 35th day it increased by 21,6 %.
Vicasol increases the activity of the Krebs cycle dehydrogenase, in particular, succinate dehydrogenase, which activity is higher during the experiment by 14- (78.2%; p≤0.05), 21- (263.3%; p≤0.05), 28- (78, 8%; p≤0.05) and the 35th (92.3%; p≤0.05) day. 2-oxoglutarate dehydrogenase activity are more specific and significantly increases relative to the control group by 14- (122.2%; p≤0.05), 21- (84.4%; p≤0.05) and on the 28th (133.3%; p≤ 0.05) day relative to the control group.
In general, vicasol activates the system of antioxidant protection and energy metabolism, that is, it is a complex activator of the body’s metabolic functions.
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