The effect of vanadium citrate on the activity of carbohydrate metabolism enzymes in pancreas and thigh skeletal muscle of pregnant female rats
DOI:
https://doi.org/10.31548/bio2019.05.004Keywords:
pregnancy, carbohydrate metabolism, vanadium citrate, pancreas, musclesAbstract
During pregnancy, the body undergoes physiological, endocrine and metabolic changes. There is an imbalance of trace elements, including vanadium. To compensate for the loss of this trace element and to improve metabolic processes in pregnant women an organic compound - vanadium citrate was used. The aim of our research was to investigate the effect of vanadium citrate on glucose-6-phosphate dehydrogenase and lactate dehydrogenase activity in pancreas and the skeletal muscle in pregnant female rats.
The research was conducted on female white laboratory rats weighing 140-160 g, which were divided into five groups: group I - non-pregnant animals, group II - pregnant females consuming pure water without additives, rats of groups III, IV, V in the period of mating and pregnancy received the solution of vanadium citrate in concentrations of 3,75, 15,625 та 62,5 mkg V/kg body weight, respectively. The material for the study included homogenates of pancreas and the skeletal muscle of the thigh of pregnant female rats, in which glucose-6-phosphate dehydrogenase and lactate dehydrogenase activity was determined.
Glucose-6-phosphate dehydrogenase activity in pancreas and skeletal muscle of pregnant female rats reduced as compared to non-pregnant rats. This is due to the inhibition of glucose oxidation in the pentose phosphate pathway. Lactate dehydrogenase activity in these tissues in pregnant animals increased as compared to group I. The increase in the activity of lactate dehydrogenase in pregnant females indicates the activation of glycolysis and the intensive use of glucose in the energy metabolism.
Under the conditions of exposure of rats to vanadium citrate, glucose-6-phosphate dehydrogenase activity in pancreas increased significantly in group IV, while lactate dehydrogenase activity decreased in group V as compared to pregnant animals of group ІІ which did not consume vanadium. In the muscles of pregnant animals exposed to vanadium, glucose-6-phosphate dehydrogenase activity significantly reduced in groups III and IV, but increased in group V, whereas lactate dehydrogenase activity decreased in group III and increased in group V as compared to pregnant animals in group II.
Vanadium contributes dose-dependently to the approximation of glucose-6-phosphate dehydrogenase and lactate dehydrogenase activity in pancreas and skeletal muscle of pregnant animals to the values of their activity in non-pregnant animals. It causes a normalizing effect on lactate dehydrogenase activity and promotes oxidation of glucose in the pentose phosphate pathway. This allows us to consider this trace element as a potential dietary supplement for pregnant women. Prospects for further studies include the study of hematological and biochemical parameters, protein metabolism in rats in different physiological and pathological conditions under the action of vanadium citrate at different concentrations.
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