Diagnosis of helmintosis in chamelions: improvement of the sedimentation method and determination of its diagnostic efficiency

Authors

  • O. V. Stets National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • N. M. Soroka National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • O. V. Semenko National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • N. A. Mezhenska State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination , Державний науково-дослідний інститут з лабораторної діагностики та ветеринарно-санітарної експертизи

DOI:

https://doi.org/10.31548/bio2019.05.018

Keywords:

helminthiasis, chameleons, diagnostics, scatological studies, sedimentation method

Abstract

The paper substantiates and experimentally confirms the need to improve the classical method of sequential washing by Demidov M. V. (1965) in the diagnosis of intestinal helminthiases of chameleons. It is established that a 15–30° degree change in the angle of the bottom of the cup during settling contributes to a
3-times increase in the concentration of helminth eggs in the test material. Optimal for filtration are 5 cm3 of the test suspension. During a microscopic examination of the material, after filtration, it is advisable to use a square central fragment of the ashless filter with a size of 25 x 25 mm. The density of the filter (tape color: blue, white, red) does not affect the number of detected helminth eggs. The diagnostic efficiency of detecting intestinal helminthiases in chameleons by the improved method is 15 % higher in comparison with classical one. The rate of invasion in Panther chameleons (Furcifer pardalis) by intestinal helminths Trematoda gen. sp. 1, Trematoda gen. sp. 2 and Hexametra angustiaecoides in the study of fecal samples with the modified sequential washing method is 15 % higher compared to the classical one. The average rate of invasion in panther chameleons (Furcifer pardalis) with intestinal helminths Trematoda gen. sp. 1, Trematoda gen. sp. 2 and
Hexametra angustiaecoides is 1.5 times higher when studying fecal samples using the improved method and is 15.5±16.19.

References

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Published

2019-12-20

Issue

Vol. 11 No. 5-6 (2019)

Section

Veterinary

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