Recovery and correction of the balance of microbiota of the gastrointestinal tract of rabbits, disabled as resulting from the use of antibiotics
DOI:
https://doi.org/10.31548/bio2018.03.003Abstract
The formation, preservation and correction of the balance of the microbiota of the gastrointestinal tract is a complex process in which factors of the external and internal environment participate. To solve the above problems, probiotic preparations are used. However, probiotics, which are used for the prevention and treatment of diseases of the gastrointestinal tract of rabbits, include bacteria isolated from various ecological niches. However, probiotic preparations are more effective, if they include representatives of obligate microbiota of the gastrointestinal tract of animals for which the drug is used. In connection with this, the creation of a probiotic preparation based on biologically active representatives of the obligate microbiota of the gastrointestinal tract of these animals remains topical. Goal. To investigate the ability of the strain of Lactobacillus helveticus 13/2 bacteria to restore and correct the balance of the microbiota of the gastrointestinal tract of young rabbits disrupted by the use of antibiotics. Methods. Microbiological and statistical. Results. Restoration of microbiota balance of the gastrointestinal tract of rabbits, impaired due to the introduction of antibiotics began in the first day after addition of the strain Lactobacillus helveticus 13/2 bacterial suspension to drinking water. The bacteria titer was 1 × 108 CFU / ml. The highest observed efficiency of administration of high doses (50 and 100 ml / l) of the bacterial suspension, provided the positive dynamics of obligate (lactic acid bacteria, bifidobacteria, E. coli) and reducing the number of optional microbiota (yeast-like fungi) in experimental animals as compared with the intestine intact.
Keywords: intestinal microbiota, dysbiosis, probiotics, lactic acid bacteria, rabbits
References
Biagi, E., Candela, M., Franceschi, C., & Brigidi, P. (2011). The aging gut microbiota: new perspectives. Ageing research reviews, 10(4), 428-429. https://doi.org/10.1016/j.arr.2011.03.004
Pessione, E. ed. (2014). Interactive probiotics. Italy: CRC Press, 274. https://doi.org/10.1201/b16439
The Normal Bacterial Flora of Humans. Available at: http://textbookofbacteriology.net/index.html
Erkosar, B., & Leulier, F. (2014). Transient adult microbiota, gut homeostasis and longevity: novel insights from the Drosophila model. FEBS letters, 588(22), 4250-4257. https://doi.org/10.1016/j.febslet.2014.06.041
Ornellas, R. M. S., Santos, T. T., Arcucio, L. B., Sandes, S. H. C., Oliveira, M. M., Dias, C. V., Nicoli, J. R. (2017). Selection of Lactic Acid Bacteria with Probiotic Potential Isolated from the Fermentation Process of “Cupuaзu”(Theobroma grandiflorum). In Advances in Microbiology, Infectious Diseases and Public Health (pp. 1-16). Springer, Cham. https://doi.org/10.1007/5584_2017_5
Walker, W. A. (2013). Initial intestinal colonization in the human infant and immune homeostasis. Annals of Nutrition and Metabolism, 63(Suppl. 2), 8-15. https://doi.org/10.1159/000354907
Langhendries, J. P. (2005). Early bacterial colonisation of the intestine: why it matters. Italian Journal of Pediatrics, 31(6), 360.
Kosiewicz, M. M., Zirnheld, A. L., & Alard, P. (2011). Gut microbiota, immunity, and disease: a complex relationship. Frontiers in microbiology, 2, 180. https://doi.org/10.3389/fmicb.2011.00180
Haller, D., Antoine, J. M., Bengmark, S., Enck, P., Rijkers, G. T., & Lenoir-Wijnkoop, I. (2010). Guidance for Substantiating the Evidence for Beneficial Effects of Probiotics: Probiotics in Chronic Inflammatory Bowel Disease and the Functional Disorder Irritable Bowel Syndrome–3. The Journal of nutrition, 140(3), 690-697. https://doi.org/10.3945/jn.109.113746
Schenk, M., Mueller, C. (2008). The mucosal immune system at the gastrointestinal barrier. Best Practice and Research Clinical Gastroenterology, 22(3), 391-409. https://doi.org/10.1016/j.bpg.2007.11.002
Liйvin-Le Moal, V., Servin, A. L. (2006). The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: mucins, antimicrobial peptides, and microbiota. Clinical microbiology reviews, 19(2), 315-337. https://doi.org/10.1128/CMR.19.2.315-337.2006
Magalhaes, J. G., Tattoli, I., Girardin, S. E. (2007). The intestinal epithelial barrier: how to distinguish between the microbial flora and pathogens. In Seminars in immunology, 19(2), 106-115. https://doi.org/10.1016/j.smim.2006.12.006
Pokhilko Yu. M., Kravchenko N. O., Bozhok L.V., Ageev V.O., Dmitruk O.M. (2015). Osoblyvosti kyshkovogo mikrobocenozu molodnjaku kroliv za riznyh typiv godivli [Features of intestinal micrococoenosis of young rabbits for different types of feeding. Agricultural Microbiology]. Agricultural Microbiology. 22, 48-52.
Antipova L.V., Glotova I.A., Rogov I. A. (2009). Metody issledovanija mjasa i mjasnyh produktov [Methods of studying meat and meat products]. Modern problems of science and education. 1, 22-22.
Vlaslo V.V., Simonov M.A. Kapilnyj V.P. (2006). Zasoby pidvyshhennja rezystentnosti kurchat [Means of increasing the resistance of chickens]. Veterinary Medicine of Ukraine. 7, 42-47.
Mokrozub V.V., Lazarenko L.N., Babenko L.P., Shinkarenko L.N., Spivak N. Ya. (2012). Antybakterial'ni j imunomoduljuval'ni vlastyvosti shtamiv lakto- ta bifidobakterij za eksperymental'noi' stafilokokovoi' infekcii' [Antibacterial and immunomodulating properties of strains of lactoid bifidobacteria in experimental staphylococcal infection]. Biotechnologia Acta. 5 (2), 98-104.
Garrity, G. M. (2012). Bergey's Manual of Systematic Bacteriology: Volume One: The Archaea and the Deeply Branching and Phototrophic Bacteria. Springer Science and Business Media,1422 р.
Motulsky, H. (2014). Intuitive biostatistics: a nonmathematical guide to statistical thinking. Oxford University Press, USA. 517.
Pokhilko Yu. M., Kravchenko N. O. (2016). Vplyv seredovyshha kul'tyvuvannja na antagonistychnu aktyvnist' molochnokyslyh bakterij [Influence of cultivation environment on antagonistic activity of lactic acid bacteria]. Agricultural Microbiology. 24, 64-72.
Pokhilko Yu. M., Kravchenko, N.O. (2016). Vydilennja iz travnoi' systemy kroliv molochnokyslyh bakterij, perspektyvnyh dlja stvorennja probiotychnyh preparativ [Isolation from the digestive system of rabbits lactic acid bacteria, promising for the creation of probiotic preparations]. Bioresources and nature management. 8 (5-6), 63-66.
Pokhilko Yu. M., Kravchenko, N.O. (2017). Stijkist'' bakterij rodu lactobacillus do metabolitiv travnoi' systemy [Resistance of bacteria of the genus lactobacillus to the metabolites of the digestive system]. Microbiology and Biotechnology. 2 (38), 101-111.
Bilko I.P. (2009). Biofunkcional'na rol' normal'noi' mikroflory organizmu ljudyny [Biophunctional role of normal microflora of human organism]. Family medicine. 1, 74-75.
Klaenhammer, T. R. (1993). Genetics of bacteriocins produced by lactic acid bacteria. FEMS microbiology reviews, 12(1‐3), 39-85. https://doi.org/10.1111/j.1574-6976.1993.tb00012.x
Pablo M. A., Gaforio J. J., Gallego A. M. et al. (1999). Evaluation of immunomodulatory effects of nisin-containing diets on mice. FEMS Immunology and Medical Microbiology, 24(1), 35-42. https://doi.org/10.1111/j.1574-695X.1999.tb01262.x
Korshunov V. M., Efimov B. A., Pikina, A. P. (2000). Harakteristika biologicheskih preparatov i pishhevyh dobavok dlja funkcional'nogo pitanija i korrekcii mikroflory kishechnika [Characteristics of biological preparations and food supplements for functional nutrition and correction of intestinal microflora]. Microbiology, 3, 86-91.
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