Effectiveness useing of the intracytoplasmic sperm injection for the transmission of genetic information in the japanese quail (сoturnix japonika)
DOI:
https://doi.org/10.31548/bio2017.01.011Abstract
This study was conducted in order to introduce new methodological approaches transgenesis quail by using intracytoplasmic sperm injection (ICSI). We researched the ability to fertilization and transfection oocytes of quail via microinjection of sperm treated by Triton X-100 with using an extract sperm and CRISPR/Cas-GFP vector.
Keywords: intracytoplasmic sperm injection, embryogenesis, oocyte, sperm, microinjection, transfection, GFP, blastoderm
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