Effect of growth regulators on in vitro regenerative ability of explants of quercus robur l.

Abstract

Development of efficient in vitro mass production technology of uninfected planting material of valuable forest-forming woody plants, including English oak (Quercus robur L.), is one of the urgent tasks of today. Domestic and foreign authors note that getting stably growing in vitro culture explants of woody plants of the family Fagaceae Dumort. is quite difficult. The purpose of the research was to determine the effect of growth regulators on in vitro regenerative ability of Q. robur explants. For the studies that were carried in January and February 2016, the embryos with fragments of the endosperm of 120-year-old donor plant were used. The following study methods were exploited: biotechnological (plant tissue culture in vitro, microclonal propagation, callus culture), statistical. It was established that the effective sterilization (above 80 %) of Q. robur embryos was achieved through the use of 0.1 % HgCl2 within 15–16 minutes. The optimal conditions for induction of callus formation in leaf blade tissues of plants Q. robur with a frequency of more than 90 % and active growth were received on MS medium (T. Murashige & F. Skoog) with the addition of 1.0 mg•L-1 BAP (6-Benzyladenine) and 0.5 mg•L-1 NAA (1-Naphthylacetic acid) in an incubator and without light. An active shoots formation in plant explants in vitro was fixed on MS medium modified with 1.0 mg•L-1 2-iP (6-(ɣ,ɣ-Dimethylallylamino) purine) and 20 mg•L-1 of adenine. Further studies are aimed at obtaining stably growing in vitro cultures of Q. robur for mass microclonal propagation

Keywords: Quercus robur L., plant tissue culture in vitro, embryo culture in vitro, explants, sterilization, culture medium, microclonal propagation, callus, shoots in vitro.