Influence of application of «Analcim-Si» and «Sporo-Leks» on indicators of nonspecific resistance of poultry

Authors

  • O. V. Machuskyy National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • V. O. Ushkalov National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • M. V. Skrypka Odessa State Agrarian University , Одеський державнийо аграрний університет
  • V. L. Kovalenko State Scientific Control Institute of Biotechnology and strains , Державний науково-контрольний інститут біотехнології і штамів мікроорганізмів
  • V. A. Machuska National Scientific Centre “ Institute of Beekeeping Named P.I. Prokopovich” , ННЦ «Інститут бджільництва ім. П. І. Прокоповича»
  • Al-Bcur Tarek Yajha Poltava State Agrarian Academy , Полтавська державна аграрна академія

DOI:

https://doi.org/10.31548/bio2018.03.026

Abstract

 Some scientists point out that montmorillonite nanostructured clays possess high sorption properties and their application in poultry farming can improve the assimilation of feed and reduce the influence of harmful microflora on the gastrointestinal system of poultry.

The goal of the work to investigate the efficiency and influence on poultry the non-specific resistance the usage of veterinary preparations "Analcim-Si" and "Sporo-leks".

The preparation "Analcim-Si" is a montmorillonite rock of the Volodymyrytske field. The fraction of the rock is from 0.01 mm to 5 mm. Probiotic "Sporo-leks" is a mix of probiotic cultures of Bacillus licheniformis VK-25 and Bacillus subtilis MK-3 on a natural standardized sorbent (montmorillonite rock of the Volodymyretske field). The efficiency of the veterinary preparations "Analcim-Si" and "Sporol-leks" was determined in laboratory, and the efficiency of the veterinary preparation "Sporo-leks" - in the field.

The study of efficiency for the poultry was carried out on chicken 130 days old cross Lohman Brown (n = 30). The bird of the first group got feed, which was added to 2% of "Analcim-Si" (20 grams of preparation per kilogram of food). Bird of the second experimental group got feed, which was added to 2% of the preparation "Sporo-leks" (20 grams of drug per kilogram of food). The third group - the control got regular food.

Hematological, biochemical and immunological parameters of blood were studied by generally accepted methods.

The results of the studies indicate a high level of correlation between the number of red blood cells and hemoglobin levels in all bird groups. Therefore, it can be argued that a significant increase in hemoglobin levels in experimental groups 1 and 2 was due to a significant increase in the number of red blood cells in the poultry blood of these groups. We associate this phenomenon with the content in the montmorillonite rock of hematite, which induces erythropoiesis.

It has been experimentally proved that the preparations of "Analcim-Si" and "Sporol-leks" significantly increase the level of BASK poultry.

Regarding changes in the dynamics of the phagocytic pox leukocyte index, there was a similar FAL-dependent relationship: only in group 2 was its significant increase compared with control (probability level 99.9%). But in group 1 the level of probability of increasing the phagocytic index of leukocytes in comparison with control was less than or equal to 64%.

Determination of lysozyme activity (muramidase test) was performed using the Micrococcus lysodekticus test strain. The obtained results indicate a significant increase in the lysozyme activity of blood serum of poultry 1 and 2 in comparison with control poultry, which makes it possible to assert - both preparations equally affect this indicator, increasing the resistance of the organism.

It is established that according to the state of morphological and biochemical parameters of blood, it is advisable to use the preparations "Analcim-Si" and "Sporo-leks" as a feed biological additive directed adaptogenic and immunocorrective action on poultry fattening.

Experimentally proved their efficiency increase in non-specific resistance of animals’ organism, by increasing the number of erythrocytes, BASK, FAL, lysozyme activity of blood serum.

Keywords: «Analcim-Si», «Sporo-leks», probiotic, efficiency, hematological and biochemical blood parameters, poultry

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Published

2018-08-14

Issue

Vol. 10 No. 3-4 (2018)

Section

Veterinary

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