MODIFICATION OF TRANSDUSER SURFACE OF BIOSENSORS ON THE BASIS OF PPR FOR DIAGNOSTICS OF LEUCOSE CATTLE
DOI:
https://doi.org/10.31548/biologiya2018.287.100Abstract
Leucosis of BLV is a widespread chronic disease of the tumor nature, occurs asymptomatically or is characterized by lymphocytosis and growth of lymphoid cells in various organs of animals. Such a disease brings huge losses in agricultural holdings. The main measure for overcoming this disease is timely diagnosis and treatment. Common methods of diagnosing leukemia are the methods of RID and ELISA. However, these methods are long-term and require research in the laboratory. Therefore, at present, it is urgent to develop new instruments for rapid diagnosis of the condition of animals in the field. The aim of the work was to work out the main approaches to the functionalization of transducer surfaces to increase the biosensor signal for the diagnosis leucosis of BLV . Immunological and statistical methods of research were used in the work. By comparing the signals of the biosensor based on PPR after its functionalization with recombinant antigens gp51 and p24, it was established that the creation of an intermediate layer on the surface of the transducer in general, and especially when using PAA (level with dodecanthiol) allows not only to increase the response, but also to achieve its necessary reproducibility on for at least 4 months. Derivatives of dextran also increase the recall of the biosensor, but do not ensure its stability during storage of the functionalized surface.
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