Regulation of technological activity of Bacillus Thuringiensis Strains 87-15 in conditions of deep cultivation

Authors

  • Т. І. Patyka National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • N. V. Patyka National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/biologiya2019.03.027

Keywords:

entomopathogenic strain, Bacillus thuringiensis, productivity, manufacturability.

Abstract

The scientific substantiation and the decision of the actual tasks of control and regulation of technological activity of the prospective entomopathogenic strain Bacillus thuringiensis var. thuringiensis (H1) 87-15 natural type as a producer of liquid form of the preparation for the biocontrol of phytophagous. Influence of different nutrient media on the productivity of strain № 87-15 at deep cultivation and the positive dynamics of spore formation and entomotoxic metabolites (titer of spores after 72 hours of fermentation in the range of 3.6-4.5 billion per ml of culture fluid) was established. The optimization of the pH of the medium, the temperature ranges and the aeration regime during the formation of the microbial mass of Bacillus thuringiensis makes it possible to obtain more biomaterial (liquid concentrate) and accelerate the process of obtaining the form of the preparation.

Author Biographies

  • Т. І. Patyka, National University of Life and Environmental Sciences of Ukraine

    доктор сільськогосподарських наук, професор кафедри молекулярної біології, мікробіології та біобезпеки

  • N. V. Patyka, National University of Life and Environmental Sciences of Ukraine

    доктор сільськогосподарських наук, професор,

    член-кореспондент НААН, завідувач кафедри

    екобіотехнології та біорізноманіття

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Published

2019-09-30

Issue

Vol. 10 No. 3 (2019)

Section

Статті

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