Genotoxic effect of Pseudomonas Syringae Pv. Atrofaciens Lipopolysaccharide In Allium Cepa-test

Authors

  • L.M. Butsenko Institute of Microbiology and Virology D.K. Zabolotnogo NAS of Ukraine , Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України
  • L.A. Pasichnyk Institute of Microbiology and Virology D.K. Zabolotnogo NAS of Ukraine , Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України
  • Y.V. Kolomiiets National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/biologiya2019.03.044

Keywords:

phytopathogenic bacteria, lipopolysaccharide, mutagenic activity, mitotic index

Abstract

The genotoxic activity of microorganisms and their biopolymers is closely monitored due to the correlation between microorganism instability of macroorganisms and carcinogenesis. Information about the mutagenic activity of phytopathogenic bacteria and their biopolymers is extremely limited, which makes the study of the genotoxic action of lipopolysaccharides of the widespread species P. syringae pv. atrofaciens urgent task. Determination of genotoxic activity of lipopolysaccharides obtained by extraction with sodium chloride solution of the causative agent of basal bacteriosis of wheat P. syringae pv. atrofaciens were performed in Allium cepa-test. It has been established that lipopolysaccharides of phytopathogenic bacteria of the species P. syringae pv. atrofaciens suppress mitotic activity in the cells of the A. cepa apical meristem and, at a dose of 5.0 mg / ml, exhibit a destructive effect on the genetic apparatus of the test object.

Author Biographies

  • L.M. Butsenko, Institute of Microbiology and Virology D.K. Zabolotnogo NAS of Ukraine, Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України
    старший науковий співробітник
  • L.A. Pasichnyk, Institute of Microbiology and Virology D.K. Zabolotnogo NAS of Ukraine, Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України
    старший науковий співробітник
  • Y.V. Kolomiiets, National University of Life and Environmental Sciences of Ukraine

    доцент кафедри екобіотехнології та біорізноманіття

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Published

2019-09-30

Issue

Vol. 10 No. 3 (2019)

Section

Статті

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