THE INFLUENCE OF N-METHYL-N′-NITRO-N-NITROSOGUANIDINE ON AVERMECTIN SYNTHESIS ABILITY OF STREPTOMYCES AVERMITILIS UKM AS-2179 AND APPEARING AUXOTROPHIC MUTANTS
DOI:
https://doi.org/10.31548/biologiya15(2).2024.001Keywords:
Streptomyces avermitilis, avermectins, N-methyl-N-nitro-N-nitrosoguanidine, mutant.Abstract
An important way to transition to ecologically safe agrotechnologies is the abandonment of chemical means of plant protection and the transition to biological ones. The effectiveness of the use of chemical means of plant protection is gradually decreasing, which is connected with the emergence of resistant populations of pests and pathogens. The aim of the presented work was to study the influence of N-methyl-N-nitro-N-nitrosoguanidine on the survival and isolation of highly active variants of S. avermitilis UKM As-2179, the avermectin-synthesizing activity of which would be higher than that of the original strain.
Biochemical methods of determining avermectin-synthesizing activity were used in the work, the qualitative assessment of the culture's ability to synthesize avermectins was performed by the TLC method, and the concentration of avermectins in ethanol extracts was determined by the colorimetric method. The influence of N-methyl-N-nitro-N-nitrosoguanidine on the viability and antibiotic activity of the avermectin complex producer S. avermitilis UKM As-2179 was studied. It was found that the optimal time for obtaining clones with increased antibiotic activity is 15 minutes of treatment with N-methyl-N-nitro-N-nitrosoguanidine, during which 30% of the spores of the studied strain survive, and the biosynthetic activity increases by 2.7 times. To identify clones of S. avermitilis UKM As-2179 with increased synthesis of natural avermectins, the method of induced МННГ mutagenesis can be used.
References
Saccá M.L., Barra Caracciolo A., Di Lenola M., Grenni P. Ecosystem services provided by soil microorganisms. In: Lukac, M., Grenni, P., Gamboni, M. (eds) Soil Biological Communities and Ecosystem Resilience. Sustainability in Plant and Crop Protection. Springer, Cham. 2017. https://doi.org/10.1007/978-3-319-63336-7_2
De Jesus Sousa JA, Olivares FL. Plant growth promotion by streptomycetes: ecophysiology, mechanisms and applications. Chem. Biol. Technol. Agric. 2017; 24:239–252. DOI:10.1186/s40538-016-0073-5
Dholakiya RN, Kumar R, Mishra A, Mody KH, Jha B. Antibacterial and antioxidant activities of novel Actinobacteria strain isolated from Gulf of Khambhat, Gujarat. Front Microbiol. 2017; 8:2420. doi:10.3389/fmicb.2017.02420
Golinska P, Wypij M, Agarkar G, Rathod D, Dahm H, Rai M. Endophytic actinobacteria of medicinal plants: diversity and bioactivity. Antonie Van Leeuwenhoek. 2015;108(2):267-89. doi: 10.1007/s10482-015-0502-7.
Harir M, Bendif H, Bellahcene M, Fortas and Rebecca Pogni Z. Streptomyces Secondary Metabolites [Internet]. Basic Biology and Applications of Actinobacteria. IntechOpen; 2018. Available from: http://dx.doi.org/10.5772/intechopen.79890
Alam K, Mazumder A, Sikdar S, Zhao Y-M, Hao J, Song C, Wang Y, Sarkar R, Islam S, Zhang Y, Li A. Streptomyces: The biofactory of secondary metabolites. Front. Microbiol. 2022, 13:968053. doi: 10.3389/fmicb.2022.968053
Klementz D, Döring K, Lucas X, Telukunta KK, Erxleben A, Deubel D, Erber A, Santillana I, Thomas OS, Bechthold A, Günther S. StreptomeDB 2.0--an extended resource of natural products produced by streptomycetes. Nucleic Acids Res. 2016; 4;44(D1):D509-14. doi: 10.1093/nar/gkv1319.
Valli S, Suvathi SS, Aysha OS, Nirmala P, Vinoth KP, Reena A. Antimicrobial potential of Actinomycetes species isolated from marine environment. Asian Pac J Trop Biomed. 2012; 2(6):469-73. doi: 10.1016/S2221-1691(12)60078-1.
Bilyavska LO. Actinobacteria of the genus Streptomyces and their metabolites in the bioregulation of plants: dissertation. ... doc. biological Sciences: 03.00.07. Kyiv, 2018. 485 p.
Jones GH. Streptomyces RNases – Function and impact on antibiotic synthesis. Front. Microbiol. 2023; 14:1096228. doi: 10.3389/fmicb.2023.1096228
Cheng J, Park SB, Kim SH, Yang SH, Suh JW, Lee CH, Kim JG. Suppressing activity of staurosporine from Streptomyces sp. MJM4426 against rice bacterial blight disease. J Appl Microbiol. 2016; 120(4):975-85. doi: 10.1111/jam.13034
Hamedi J, Mohammadipanah F. Biotechnological application and taxonomical distribution of plant growth promoting actinobacteria. J Ind Microbiol Biotechnol. 2015; 42(2):157-71. doi: 10.1007/s10295-014-1537-x.
Iutynska GO, Tytova LV, Leonova NO, Antypchuk AF, Brovco IS, Eakin D, Valahurova HV, Kozyritska VE, Biliavska LO, Petruk TV, Ponomarenko S.P, Yamborko NA, Pindrus AA Complex preparations based on microorganisms and plant growth regulators. In: Iutynska GO, Ponomarenko SP (eds). New plant growth regulators: basic research and technologies of application. Kyiv: Nichlava; 2011:161-208.
Pastor V, Luna E, Mauch-Mani B, Ton J, Flors V. Primed plants do not forget, Environmental and Experimental Botany. 2013; 94:46-56. https://doi.org/10.1016/j.envexpbot.2012.02.013.
El-Saber Batiha G, Alqahtani A, Ilesanmi OB, Saati AA, El-Mleeh A, Hetta HF, Magdy Beshbishy A. Avermectin Derivatives, Pharmacokinetics, Therapeutic and Toxic Dosages, Mechanism of Action, and Their Biological Effects. Pharmaceuticals (Basel). 2020;13(8):196. doi: 10.3390/ph13080196.
Wang L., Yan X., Li Y, Gao C, Liu J. Preparation and Characterization of Avermectin B2 Microcapsules and Effective Control of Root-Knot Nematodes. ACS Omega. 2023; 8(14): 13038-13047. DOI: 10.1021/acsomega.3c00244
Iutynska GO, Biliavska LO, Kozyritska VE. Development strategy for the new environmentally friendly multifunctional bioformulations based on soil streptomycetes. Micro. J. 2017; 79(1):22-33. DOI:10.15407/microbiolj79.01.022
Petruk TV, Biliavs'ka LO, Kozyryts'ka VIe, Mukvych MS. Increasing biosynthesis of avermectins of Streptomyces avermitilis UCM Ac 2161 under the effect N-methyl-N'-nitro-N-nitrosoguanidine. Mikrobiol Z. 2004; 66(6):24-30. Ukrainian. PMID: 15765867.
Dashwood RH. Cancer interception by interceptor molecules: mechanistic, preclinical and human translational studies with chlorophylls. Genes and Environ. 2021; 43:8. https://doi.org/10.1186/s41021-021-00180-8
Martı́n JF. Molecular mechanisms for the control by phosphate of the biosynthesis of antibiotics and other secondary metabolites. Regulation of Secondary Metabolism in Actinomycetes. 2020:213-237. https://doi.org/10.1201/9781003068600-6
Vinogradov AA, Suga H. Introduction to thiopeptides: biological activity, biosynthesis, and strategies for functional reprogramming. Cell Chemical Biology. 2020; 27(8):1032-1051. https://doi.org/10.1016/j.chembiol.2020.07.003.
Chen J, Liu M, Liu X, Miao J, Fu C, Gao H, Müller R, Zhang Q, Zhang L. Interrogation of Streptomyces avermitilis for efficient production of avermectins. Synthetic and Systems Biotechnology. 2016; 1(1):7-16. https://doi.org/10.1016/j.synbio.2016.03.002.
Alef K, Nannipieri P. Soil respiration. Methods in applied soil microbiology and biochemistry. New York: Academic Press; 1995. 214–218.
Fedorenko VO, Ostash BO, Gonchar MV, Rebets YuV. Large workshop on genetics, genetic engineering and analytical biotechnology of microorganisms. Lviv: Ivan Franko LNU Publishing Center, 2007. 279 p.
Rütten A, Kirchner T, Musiol-Kroll EM. Overview on Strategies and Assays for Antibiotic Discovery. Pharmaceuticals. 2022; 15(10):1302. https://doi.org/10.3390/ph15101302
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