DETERMINATION OF GENOTOXICITY OF NANOCOMPOSITES USING SOSTYPE BIOSENSOR

Authors

  • M. Savchuk National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • M. Fedelesh-Gladinets National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • M. Starodub National University of Life and Environmental Sciences of Ukraine image/svg+xml

Abstract

In recent years in Ukraine and the world there has been a rapid development of a new branch of science – nanotechnology. Due to their size, shape and properties, nanoparticles have received a great demand in medicine, food industry, machine building, agriculture. But along with many positive aspects of nanomaterials, scientists also warn about their danger with respect to living organisms, so studying the biological effects of nanomaterials is a must before using them. Biosensor diagnostic methods have become very popular, since these devices can quickly and easily estimate the level of toxicity of foreign agents. The genotoxicity of nanocomposites based on saponite with the help of SOS-type biosensors based on fiber optics was investigated. SOS – the biosensor operates on the basis of recording the damage of bacterial DNA, restored by the reparation systems of bioluminescent bacteria. According to the results of the research it was shown that newly synthesized nanocomposites based on saponite do not possess genotoxicity in the concentration range of 300-600mg/l. Nano-sized SiO2, which is a composite nanocomposite, demonstrated genotoxicity relative to the reference culture in the concentration range of 300-600mg/l.

Author Biographies

  • M. Savchuk, National University of Life and Environmental Sciences of Ukraine
    молодший науковий співробітник
  • M. Fedelesh-Gladinets, National University of Life and Environmental Sciences of Ukraine
    кандидат сільськогосподарських наук, доцент кафедри молекулярної біології, мікробіології та біобезпеки
  • M. Starodub, National University of Life and Environmental Sciences of Ukraine
    доктор біологічних наук, професор, завідувач кафедри молекулярної біології, мікробіології та біобезпеки

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Published

2018-02-20

Issue

No. 270 (2017)

Section

Статті

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