FORMATIVE ACTIVITY UNDER THE ACTION OF IONIZING RADIATION FOR WINTER WHEAT AT THE CELL LEVEL
DOI:
https://doi.org/10.31548/biologiya13(3-4).2022.028Keywords:
bread wheat, ionizing radiation, cytogenetic activity, chromosomal rearrangements.Abstract
The purpose of the conducted experiments was to show the cytogenetic activity of a wide range of doses of ionizing radiation in individual genotypes of common wheat at the level of the chromosomal apparatus of the cell. In the study, seeds of winter wheat varieties Podolyanka and Smuglyanka were used, irradiated with gamma rays in doses of 100, 150, 200, 250, 300 Gy. The control was seeds without treatment.
Based on the data of cytological analysis, the frequencies and spectra of chromosomal aberrations after exposure to gamma rays were studied. The total number of mitoses (in the corresponding phase) found in the preparations (20 - 25 preparations for each variant), the number of cells with chromosomal abnormalities and the percentage of such cells (from the number of mitotic), the frequency of chromosomal aberrations (from the total number of cells with rearrangements). The sample was approximately 500 - 1000 cells for each study variant.
Variety Smuglyanka is significantly less stable compared to the variety Podolyanka at the cytogenetic level, with the presence of significant differences in the interaction of the genotype-mutagen system for gamma rays. The number of chromosomal rearrangements increases linearly under the influence of gamma rays up to 200 - 250 Gy, where a significant drop begins with stabilization at a lower level at doses of 250 - 300 Gy. It was found that the dose of the mutagen is a significantly more significant factor of influence, but the nature of the genotype is also important. Significant variability parameters are the total frequency of chromosomal aberrations, the frequency of micronuclei and lagging chromosomes, the frequency of bridges, and the frequency of complex rearrangements. The ratio of fragments to bridges is standard for gamma rays. A higher level of variability in subsequent generations is foreseen for the variety Smuglyanka, the possibility of differences in the spectrum of changes in subsequent generations due to the action of gamma rays.
References
Fathin, T., Hartati, S., & Yunus, A. (2021). Diversity induction with gamma ray irradiation on Dendrobium odoardi orchid. IOP Conference Series: Earth and Environmental Science, 637. 012035. doi: 10.1088/1755-1315/637/1/012035
Holme, I., Gregersen, P., & Brinch-Pedersen, H. (2019). Induced genetic variation in crop plants by random or targeted mutagenesis: convergence and differences. Frontiers in Plant Science, 10. 1468. doi: 10.3389/fpls.2019.01468
Nazarenko M. (2016) Characteristics of action of nitrosoalkylureas on cell level in winter wheat. Visnyk of Dnipropetrovsk University. Biology, ecology. 24(2), 258–263. doi:10.15421/011632
Nazarenko M.M. & O. O. Izhboldin (2017) Chromosomal rearrangements caused by gamma-irradiation in winter wheat cells. Biosystems Diversity. 25(1). 25–28. doi: 10.15421/011704
Nurmansyah, S., Alghamdi, S., Hussein, M., & Farooq, M. (2018). Morphological and chromosomal abnormalities in gamma radiation-induced mutagenized faba bean genotypes. International Journal Radiation Biology, 94(2). 174-185. doi: https://doi.org/10.1080/09553002.2018.1409913
Nikolova, I., Georgieva, M., Kruppa, K., Molnor-Long, M., Liu, L., Manova, V., & Stoilov, L. (2015). Cytogenetic effects in barley root apical meristem after exposure of dry seeds to lithium ion beams. Genetics and Plant Physiology, 5, 3–9. http://www.bio21.bas.bg/ippg/bg/wp-content/uploads/2015/04/GPP_5_1_2015_03-09.pdf
Oney-Birol, S. & Balkan, A. (2019). Detection of Cytogenetic and Genotoxic Effects Of Gamma Radiation on M1 Generation of Three Varieties of Triticum aestivum L., Pakistan Journal of Botany, 51(3), 887–894. doi: 10.30848/PJB2019-3(48)
Shu, Q.Y., Forster, B.P. & Nakagava, H., (2013). Plant mutation breeding and biotechnology. CABI publishing, Vienna. doi: 10.1079/9781780640853.0000.
Spencer-Lopes, M.M., Forster, B.P. Jankuloski L. (2018). Manual on mutation breeding. Third edition. Food and Agriculture Organization of the United Nations, Rome.
Yali, W., & Mitiku, T. (2022). Mutation Breeding and Its Importance in Modern Plant Breeding. Journal of Plant Sciences, 10(2), 64–70. doi: 10.11648/j.jps.20221002.13
Downloads
Published
Issue
Section
License
Relationship between right holders and users shall be governed by the terms of the license Creative Commons Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
