Sensitivity of sorghum bacteriosis to antibiotics

Authors

  • М. Reshtnikov graduate student of the Department of Phytopathogenic Bacteria Institute of Microbiology and Virology named after D.K. Zabolotny National Academy of Sciences of Ukraine , аспірант відділу фітопатогенних бактерій Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України, Київ, Україна
  • S. Moroz senior researcher of the department of phytopathogenic bacteria Institute of Microbiology and Virology named after D.K. Zabolotny National Academy of Sciences of Ukraine , старший науковий співробітник відділу фітопатогенних бактерій, Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України, Київ, Україна
  • L. Pasichnyk senior researcher of the Department of Phytopathogenic Bacteria Institute of Microbiology and Virology named after D.K. Zabolotny National Academy of Sciences of Ukraine , старший науковий співробітник відділу фітопатогенних бактерій Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України, Київ, Україна
  • L. Butsenko professor of the Department of Biotechnology and Microbiology National University of Food Technologies , професор кафедри біотехнологій і мікробіології Національний університет харчових технологій, Київ, Україна

DOI:

https://doi.org/10.31548/biologiya14(1-2).2023.003

Keywords:

bacterial diseases, sorghum, phytopathogenic bacteria, resistance to antibiotics

Abstract

The significant spread of antibiotic resistance, which is a global human problem,
 is largely due to the excessive use of antibiotics in agricultural practices. 
The increase in the number of antibiotics in crop production, which is associated with both direct 
use for the control of phytopathogens and the arrival together with livestock wastes used as fertilizers, 
leads to an increase in resistance to antimicrobial drugs among plant-associated microorganisms. 
At the same time, in Ukraine there are practically no data on the detection of antibiotic-resistant strains 
in crop production and its products, and control over the circulation of bacteria with acquired resistance in this area. 
The purpose of the study is to determine the sensitivity of strains of bacterial pathogens of sorghum P. syringae to antimicrobial 
drugs with different mechanisms of action.
Determination of antibiotic sensitivity was carried out by the disk diffusion method among strains of Pseudomonas syringae isolated 
from sorizia and strains of phytopathogenic bacteria from the collection of the department of phytopathogenic bacteria of the Institute of 
Microbiology and Virology named after D.K. Zabolotny NASU. It was established that the studied strains were divided into 5 groups according 
to resistance to antimicrobial substances. The most common is resistance to cephalosporin antibiotics. Freshly isolated strains isolated from 
soriza showed a spectrum of antibiotic sensitivity similar to the collection strains of P. syringae. The studied strains of P. syringae were most 
sensitive to streptomycin, tetracycline, and chloramphenicol. One of the potential sources for the search for active antagonists against these
 pathogens is soil streptomycetes.

References

Mann, A., Nehra, K., Rana, J. S., Dahiya, T. (2021). Antibiotic resistance in agriculture: Perspectives on upcoming strategies to overcome upsurge in resistance. Current research in microbial sciences. 2. 100030. https://doi.org/10.1016/j.crmicr.2021.100030.

Iwu, C. D., Korsten, L., Okoh, A. I. (2020). The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health. MicrobiologyOpen. 9(9), e1035. https://doi.org/10.1002/mbo3.1035.

World Health Organization (WHO) (2017). Antimicrobial resistance in the food chain. [WWW Document]. WHO. Retrieved from https://www.who.int/foodsafety/areas_work/antimicrobial‐resistance/amrfoodchain/en/.

Wang, F.‐H., Qiao, M., Chen, Z., Su, J.‐Q., Zhu, Y.‐G. (2015) Antibiotic resistance genes in manure‐amended soil and vegetables at harvest. Journal of Hazardous Materials. 299, 215–221. 10.1016/j.jhazmat.2015.05.028.

Buletsa, N.M., Butsenko, L.M., Pasichnyk, L.A., Patyka, V.P. (2015). The sensitivity of phytopathogenic bacteria to streptomycin under the action of pesticides. Journal of microbiology. 77(6); 62–69.

Buletsa, N.M., Butsenko, L.M., Pasichnyk, L.A., Patyka, V.P. (2015). The influence of pesticides on the antibiotic sensitivity of phytopathogenic bacteria. 5th All-Ukrainian congress of ecologists with international participation. September 23-26, 2015. Vinnytsia: Collection of Sciences. works - Vinnytsia, 130.

Sundin, G. W., Wang, N. (2018) Antibiotic Resistance in Plant-Pathogenic Bacteria. Annual review of phytopathology. 56, 161–180. https://doi.org/10.1146/annurev-phyto-080417-045946.

Methodical recommendations for determining the sensitivity of microorganisms to antimicrobial drugs (2021): Methodical recommendations /T.O. Harkavenko, O.I. Horbatyuk, T.G. Kozytska, V.O. Andriyashchuk, V.M. Harkavenko, S.M. Dybkova, I.V. Azyrkina - K: DNDILDVSE,. 101 p. http://vetlabresearch.gov.ua/derzhavnizakupivli/docs/%D0%90%D0%BD%D1%82%D0%B8%D0%B1%D1%96%D0%BE%D1%82%D0%B8%D0%BA%D0%BE%D1%80%D0%B5%D0%B7%D0%B8%D1%81%D1%82%D0%B5%D0%BD%D1%82%D0%BD%D1%96%D1%81%D1%82%D1%8C.pdf

https://likicontrol.com.ua/%D1%96%D0%BD%D1%81%D1%82%D1%80%D1%83%D0%BA%D1%86%D1%96%D1%8F/?%5b34408

Vasebia, Y., Khakvara, R., Faghihib, M.M., Vinatzerc, B.A. (2019). Genomic and pathogenic properties of Pseudomonas syringae pv. syringae strains isolated from apricot in East Azerbaijan province, Iran. Biocatalysis and Agricultural Biotechnology. Volume 19, May, 101167 https://doi.org/10.1016/j.bcab.2019.101167.

Burkot, V.M. (2021). Characterization of the influence of environmental factors on the biological activity of gram-negative non-fermenting bacteria. Dissertation for obtaining the degree of Doctor of Philosophy. Vinnytsia National Medical University named after M. I. Pirogov, Ministry of Health of Ukraine, Vinnytsia. 149.

Hossain, M.F., Hasan, S.M.Z., Zaoti, Z.F., Hasan, M.F., Acharjee, U.K., Islam, M.A., Khalekuzzaman, M., Sikdar, B. (2017). Isolation and characterization of Pseudomonas syringae pv. lachrymans from angular leaf spot disease of cucumber (Cucumis sativus L.) and evaluation of its antibiotic sensitivity. Journal of Pharmacognosy and Phytochemistry. 6(6): 233–238.

Aćimović, S.G., Zeng, Q., McGhee, G.C., Sundin, G.W., Wise, J.C. (2015). Control of fire blight (Erwinia amylovora) on apple trees with trunk-injected plant resistance inducers and antibiotics and assessment of induction of pathogenesis-related protein genes. Front. Plant Sci., 10 February https://doi.org/10.3389/fpls.2015.00016.

Serizawa, S, Ichikawa, T, Takikawa, Y, Tsuyumu, S, Goto, M. (1989). Occurence of bacterial canker of kiwifruit in Japan: description of symptoms, isolation of the pathogen and screening of bactericides. Annals of the Phytopathological Society of Japan. 55, 427–36.

Han, H.S, Nam, H.Y, Koh, Y.J, Hur, J.-S., Jung, J.S. (2003). Molecular bases of high-level streptomycin resistance in Pseudomonas marginalis and Pseudomonas syringae pv. actinidiae. Journal of Microbiology. 41, 16–21.

Nakajima, M., Yamashita, S., Takikawa, Y., Tsuyumu, S., Hibi, T., Goto, M. (1995). Similarity of streptomycin resistance gene(s) in Pseudomonas syringae pv. actinidiae with strA and strB of plasmid RSF1010. Annals of the Phytopathological Society of Japan 61, 489–92.

Moragrega, C., Manceau, C., Montesinos, E. (1998). Evaluation of drench treatments with phosphonate derivatives against Pseudomonas syringae pv. syringae on pear under controlled environment conditions. European Journal of Plant Pathology. 104: 171–180

Bulygina, T. V., Varbanets, L. D., Pasichnyk, L. A., Zhitkevich, N. V. (2016). Antimicrobial resistance of Pantoea agglomerans bacteria. Microbiology and biotechnology. No. 1. 68–75. http://nbuv.gov.ua/UJRN/MiB_2016_1_9.

Published

2023-08-21

Issue

Section

Статті