The utility of metagenomic data for the black sea ecological status assessment
DOI:
https://doi.org/10.31548/biologiya14(1-2).2023.006Keywords:
ecological status, index, microbial communities, xenobiotics, Black seaAbstract
According to Marine Strategy Framework Directive the assessment of marine ecosystems’ environmental status is performed with 11 Descriptors, which are based on biological, physical and chemical parameters. The biological indexes are calculated based on taxonomic structure and distribution of eukaryotic communities. Meanwhile, bacterioplankton is sensitive to anthropogenic impact and is thus a promising indicator of aquatic environment status.
We have tested the utility of ecological pressure index (Pi) calculated with chemical data and bacterial community-based index (microgAMBI) for the assessment of Black sea waters ecological status. According to our estimations Pi varied depending on the chemical data used for its calculation, which indicates the necessity of using a wide range of xenobiotics in complex ecological status assessment. MicrogAMBI indicated that the Black sea surface waters can be characterized by “good” environmental status. “Moderate”, “poor” and “bad” ecological status was shown for 3 stations, yet there were no significant region-specific differences between the shelf zone and the open water.
Actinomycetales, Halomonadaceae and Shewanella relative abundance was associated with higher microgAMBI estimations and respectively with worse ecological status. Meanwhile, positive correlation was found between Synechococcus, Acidimicrobiaceae, Pelagibacteraceae, Rhodobacteraceae, Microbacteriaceae, Polaribacter, Rhodothermaceae and Chloroflexi abundance and “good” ecological status.
Microbial metagenomic data is promising for the complex assessmnet of Black sea waters ecological status, however, more research is needed to validate this approach. The development of metagenomic databases will contribite to increase in precision of microgAMBI calculation and subsequent ecological status analysis.
References
Water Framework Directive (Directive 2000/60/EC of the European Parliament and of the Council establishing a framework for the Community action in the field of water policy, Official Journal (OJ L 327) on 22 December 2000
Directive 2008/56/EC of the European Parliament and of the Council establishing a framework for community action in the field of marine environmental policy (Marine Strategy Framework Directive). 17 June 2008 establishing a framework for community action in the field of marine environmental policy (Marine Strategy Framework Directive) (Text with EEA relevance) (OJ L 164, 25.6.2008) 27 pp.
Caruso, G., La Ferla, R., Azzaro, M., Zoppini, A., Marino, G., Petochi, T., Corinaldesi, C., Leonardi, M., Zaccone, R., Fonda, S., Caroppo, C., Monticelli, L., Azzaro, F., Decembrini, F., Maimone, G., Cavallo, R., Stabili, L., Todorova, N., Karamfilov, V., … Danovaro, R. (2016). Microbial assemblages for environmental quality assessment: Knowledge, gaps and usefulness in the European marine strategy framework directive. In Critical Reviews in Microbiology. https://doi.org/10.3109/1040841X.2015.1087380
Aylagas, E., Borja, Á., Tangherlini, M., Dell’Anno, A., Corinaldesi, C., Michell, C. T., Irigoien, X., Danovaro, R., & Rodríguez-Ezpeleta, N. (2017). A bacterial community-based index to assess the ecological status of estuarine and coastal environments. Marine Pollution Bulletin. https://doi.org/10.1016/j.marpolbul.2016.10.050
Zhang, Y., Chen, L., Sun, R., Dai, T., Tian, J., Liu, R., & Wen, D. (2014). Effect of wastewater disposal on the bacterial and archaeal community of sea sediment in an industrial area in China. FEMS Microbiology Ecology. https://doi.org/10.1111/1574-6941.12298
Stoeck, T., Frühe, L., Forster, D., Cordier, T., Martins, C. I. M., & Pawlowski, J. (2018). Environmental DNA metabarcoding of benthic bacterial communities indicates the benthic footprint of salmon aquaculture. Marine Pollution Bulletin. https://doi.org/10.1016/j.marpolbul.2017.11.065
Laroche, O., Pochon, X., Tremblay, L. A., Ellis, J. I., Lear, G., & Wood, S. A. (2018). Incorporating molecular-based functional and co-occurrence network properties into benthic marine impact assessments. FEMS Microbiology Ecology. https://doi.org/10.1093/FEMSEC/FIY167
Lanzén, A., Mendibil, I., Borja, Á., & Alonso-Sáez, L. (2021). A microbial mandala for environmental monitoring: Predicting multiple impacts on estuarine prokaryote communities of the Bay of Biscay. Molecular Ecology. https://doi.org/10.1111/mec.15489
Slobodnik, J., Alexandrov, B., Komorin, V., Mikaelyan, A., Guchmanidze,A., Arabidze, M., Korshenko, A. National Pilot Monitoring Studies and Joint Open Sea Surveys in Georgia, Russian Federation and Ukraine, 2017: Final Scientific Report / J. Slobodnik, B. Alexandrov, V. Komorin, A. Mikaelyan, A. Guchmanidze, M. Arabidze, A. Korshenko. – Dnipro: Seredniak T.K., 2020b
Directive 2013/39/EU of the European Parliament and of the Council of 12 August 2013 amending Directives 2000/60/EC and 2008/105/EC as regards priority substances in the field of water policy Text with EEA relevance (OJ L 226 24.08.2013, p. 1, ELI: http://data.europa.eu/eli/dir/2013/39/oj)
Borja, A., Franco, J., & Pérez, V. (2000). A marine Biotic Index to establish the ecological quality of soft-bottom benthos within European estuarine and coastal environments. Marine Pollution Bulletin. https://doi.org/10.1016/S0025-326X(00)00061-8
Borja, A. (2018). Testing the efficiency of a bacterial community-based index (microgAMBI) to assess distinct impact sources in six locations around the world. Ecological Indicators. https://doi.org/10.1016/j.ecolind.2017.11.018
Hamedi, J., Mohammadipanah, F., & Ventosa, A. (2013). Systematic and biotechnological aspects of halophilic and halotolerant actinomycetes. In Extremophiles. https://doi.org/10.1007/s00792-012-0493-5
Devanshi, S., R. Shah, K., Arora, S., & Saxena, S. (2022). Actinomycetes as An Environmental Scrubber. In Crude Oil - New Technologies and Recent Approaches. https://doi.org/10.5772/intechopen.99187
Kalaitzidou, M. P., Alvanou, M. V., Papageorgiou, K. V., Lattos, A., Sofia, M., Kritas, S. K., Petridou, E., & Giantsis, I. A. (2022). Pollution Indicators and HAB-Associated Halophilic Bacteria Alongside Harmful Cyanobacteria in the Largest Mussel Cultivation Area in Greece. International Journal of Environmental Research and Public Health. https://doi.org/10.3390/ijerph19095285
Dong, C., Bai, X., Sheng, H., Jiao, L., Zhou, H., & Shao, Z. (2015). Distribution of PAHs and the PAH-degrading bacteria in the deep-sea sediments of the high-latitude Arctic Ocean. Biogeosciences. https://doi.org/10.5194/bg-12-2163-2015
Pepi, M., Heipieper, H. J., Balestra, C., Borra, M., Biffali, E., & Casotti, R. (2017). Toxicity of diatom polyunsaturated aldehydes to marine bacterial isolates reveals their mode of action. Chemosphere. https://doi.org/10.1016/j.chemosphere.2017.03.031
Catania, V., Cappello, S., Di Giorgi, V., Santisi, S., Di Maria, R., Mazzola, A., Vizzini, S., & Quatrini, P. (2018). Microbial communities of polluted sub-surface marine sediments. Marine Pollution Bulletin. https://doi.org/10.1016/j.marpolbul.2018.04.015
Lemaire, O. N., Méjean, V., & Iobbi-Nivol, C. (2021). The Shewanella genus: Ubiquitous organisms sustaining and preserving aquatic ecosystems. In FEMS Microbiology Reviews. https://doi.org/10.1093/FEMSRE/FUZ031
Haggerty, J. M., & Dinsdale, E. A. (2017). Distinct biogeographical patterns of marine bacterial taxonomy and functional genes. Global Ecology and Biogeography. https://doi.org/10.1111/geb.12528
Downloads
Published
Issue
Section
License
Relationship between right holders and users shall be governed by the terms of the license Creative Commons Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
