Detection of genetically modified plants in the agricultural products
DOI:
https://doi.org/10.31548/dopovidi2020.03.012Keywords:
Genetically modified organisms, Real-time PCR, GM-plantsAbstract
The use of genetically modified organisms in the production of agricultural and food products is important for the view of food security. Despite this, there is a concern in society about the possible threat of GMOs to human health and biodiversity. Nowadays the likely risks are divided into food, environmental and socio-economic risks.
There is a necessity for systematic research on the spread of GM crops and the appropriate mandatory labeling of products, which include components derived from GM plants. It gives consumers the right to choosing to eat or not GM-foods. For this reason, in many countries GMOs' circulation is regulated and controlled.
The article presents systematic data of the analysis of samples agriculture products, which were analyzed regarding the content of GMOs in the Department of molecular -biological research of Ukrainian Laboratory of Quality and Safety of Agricultural Products during 2018-2019. To conduct an analysis of the GMO content in agriculture products CFX96 Real-Time PCR (BioRad) was used. The study was conducted according to ISO 21569:2005 Foodstuffs – Methods of analysis for the detection of genetically modified organisms and derived products – Qualitative nucleic acid based methods; ISO 21570:2005 Foodstuffs – Methods of analysis for the detection of genetically modified organisms and derived products – Quantitative nucleic acid based methods; ISO 21571:2005 Foodstuffs – Methods of analysis for the detection of genetically modified organisms and derived products – Nucleic acid extraction.
In 2018 were analyzed 319 samples about GMO content and 93 (29.2%) of them were positive. Among the positive samples, 66.7% were the premixes, 11.8% – rapeseed, 9.7% – soybean meal. In 2019 was analyzed 130 samples and 9 (6.9 %) of them were found positive.
It is established that such GM plants as soybeans GTS 40-3-2, rapeseed GT 73 are present in the agricultural market of Ukraine. Furthermore they are not only actively grown, but also used for the production of feed.
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