Proteinogram of blood of animals in a state of hypobiosis during e.coli infection
DOI:
https://doi.org/10.31548/dopovidi2020.06.020Keywords:
hypobiosis, E. coli disease, disease stages, proteinogrammaAbstract
The features of fractional distribution of plasma proteins in rats with Escherichia coli at different stages of infection are established. It was studied the effect of hypobiosis on the proteinogramma under and after artificial sleep too. Artificial hypobiosis is important for therapeutic use at bacterial infection and proteinogramma is informatively significant as a diagnostic element of the functional changes provoked by a hypobiosis. We used the nephelometric method. Experiments were performed on young rats with Escherichia coli, in a state of hypobiosis and one day after leaving it. It was established the infection provoked changes in plasma proteins such as increasing globulins and decreasing albumin at the beginning of the disease and the opposite trend during the period of total depletion. The introduction of sick animals into a state of hypobiosis causes an increase in nonspecific resistance of the organism level due to an increase in the content of γ-globulin fraction and a decrease in the level of albumin, which increases the survival of animals after removal from hypobiosis. Indicators of the proteinogram of blood of animals that were introduced into the state of hypobiosis at the initial stage of the disease and the stage of progressive symptoms return to control values on the first day after the elimination of hypobiosis factors. Further research should be aimed at a comprehensive study of functional changes in the body according to the proposed model.
References
Andjus, R.К, Smith, A.U. (1956). Reanimation of adult rats from body temperatures between 0 and +2 °С. Journal of Physiology, 128(3), 446 472. https://doi.org/10.1113/jphysiol.1955.sp005318
ATCC Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™) Strain Designations: FDA strain Seattle 1946 [DSM 1103, NCIB 12210] /Type Strain: no/ Biosafety Level: 1. Available at: https://www.lgcstandards-atcc.org/products/all/25922.aspx?geo_country=ru 9.
Bettelheim, K., Goldwater P. (2004). Outbreak of Shiga Toxin-Producing Escherichia coli O111:H8 Infection. Clinical Infectious Diseases, 39(1), 148-149. https://doi.org/10.1086/421781
McFarlad Standart -For in vitro use only- Catalogue No. TM50-TM60. Available at: http://www.dalynn.com/dyn/ck_assets/files/tech/ TM53.pdf
Piercefield, E.W., Bradley, K.K., Coffman, R.L., Mallonee, S.M. (2010). Hemolytic Uremic Syndrome After an Escherichia coli O111 Outbreak. Archive International Medicine, 170(18): 1656-1663.
https://doi.org/10.1001/archinternmed.2010.346
Unilab 2020 Available at: https://www.unilab.com.ua/index.php/ua/lista-badan/product/52
Zaias, J., Mineau, M., Cray, C., Yoon, D., Altman NH. (2009). Reference values for serum proteins of common laboratory rodent strains. Journal of American Association of Laboratory Animal Sciences, 48(4):387 - 390.
Bodnar P.M., Mykhalchyshyn H.P., Komisarenko Yu.I. (2013) Endokrynolohiia: dlia studentiv vyshchykh med.navch. zakladiv [Endocrinology: for students of higher medical education. institutions]. Vinnytsia: Nova Knyha, 480.
Karpov Y.A., Astapov A.A., Sebut N.S., Semenov V.M., Dmytrochenko T.Y., Burakovskyi N.Y., Protasov S.A. (2003) Diagnostika i lecheniye ostrykh kishechnykh infektsiy. Instruktsiya po primeneniyu [Diagnosis and treatment of acute intestinal infections. Instructions for use] Belarus, 25.
Kondrakhyn, Y.P. (2004) Metody veterinarnoy klinicheskoy laboratornoy diagnostiki: Spravochnik [Methods of Veterinary Clinical Laboratory Diagnostics: A Handbook]. Moscow, Kolos, 520.
Lakin G.F. (1990) Biometriya. [Biometrics] M.: Vysshaya shkola. 352.
Makarenko T.M., Radchenko O.M. (2017). The ratio of biochemical parameters of blood in medical practice: clinical and diagnostic value. Practitioner. V. 6(2). P. 49-53.
Melnychuk S.D., Vykhovanets V.I. (2005). Influence of artificial hypobiosis conditions on energy metabolism in rats. Ukrainian Biochemical Journal. № 77 (3). P. 131-135.
Melnychuk S.D., Khyzhniak S.V., Morozova V.S., Boiko V.V., Babich L.V., Voitsitskyi V.M. (2013). Biochemical parameters of blood of rats under conditions of artificial hypobiosis. Bioresources and nature management. V.5 (3-4). P. 5-11.
Olkhovska O.M. (2011). Features of the clinical course and ways to improve the treatment of intestinal infections in children at the present stage. Annals of Mechnikov Institute. № 4. P. 258-261.
Popov V.S. (2015). Scientific and practical substantiation of means and methods of immunometabolic correction in pigs. Kursk, 274 p.
Timofeyev N.N. (1983) Iskusstvennyy gipobioz: monografiya [Artificial hypobiosis: monograph]. Moscow: Medicine, 192.
Umanska A.O., Melnychuk D.O., Kalachniuk L.H. (2017). Hematological parameters of rats with artificial hypobiosis. Scientific Journal «ScienceRise:Biological Science». №6(9). P. 7-9.
https://doi.org/10.15587/2519-8025.2017.118786
Umanska A.O., Melnychuk D.O., Melnychuk L.G., Kalachniuk L.H., Morozova D.O. (2017). Indicators of the fatty acid spectrum of serum lipids of rats in artificial hypobiosis. Animal biology. V.19(2). P. 115-120. https://doi.org/10.15407/animbiol19.02.115
Tsvilikhovskyi V.I., Tekdemir I.O., Makhendirarasa L.P. (2019). The state of hypobiosis in Escherichia coli: time frame of survival of animals and the success of the method in the early and late diagnosis of the disease. Bulletin of problems of biology and medicine. Issue 4, V.2 (154). P. 62-67.
https://doi.org/10.29254/2077-4214-2019-4-2-154-62-67
Cherkessova D.U. (2013). Physiological aspects of cellular and molecular patterns of adaptation of animal organisms to extreme situations. Astrakhan, 32 p.
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