Estimation of the biological efficiency of prospective dominant strains of bacteria in the rhizosphere of spring barley

Authors

  • M. O. Kiroiants National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • M. V. Patyka National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • T. I. Patyka National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/dopovidi2021.01.001

Keywords:

Phyllobacterium ifriqiyense 1, Bacillus methylotrophicus 10, inoculation, biometric indicators, spring barley, floratest

Abstract

An important task of modern agricultural production is the creation of new environmentally friendly agricultural technologies aimed at increasing the productivity of crops. Spring barley is an important forage crop, which occupies a significant place in the balance of concentrated feed. Therefore, our study is quite relevant today, given the trend towards the transition to organic farming and the development of biological fertilizers. The aim of the study was to determine the efficiency of inoculation with dominant strains of rhizosphere bacteria Phyllobacterium ifriqiyense 1 and Bacillus methylotrophicus 10 seeds of spring barley and physiological parameters of growth and development of the culture in model conditions. The task of the study is to determine the titer of viable bacteria, exposure of spring barley seeds in bacterial suspension, accounting for the growth of spring barley plants and fixing its biometric parameters after bacterization; determination of physiological parameters of plant growth and development in model conditions. Research methods – microbiological (direct calculation of viable colony-forming units using Goryaev's camera). Indicators of photosynthetic activity of the leaf were determined using the biophysical method of induction of chlorophyll fluorescence by a portable device "Floratest". The results of studies – the strain Phyllobacterium ifriqiyense 1 showed the maximum biological effect on the growth of test culture of barley (including dilution of culture fluid). It was found that treatment with bacterial suspensions of Phyllobacterium ifriqiyense 1 and Bacillus methylotrophicus 10 promotes the activation of the functions of the photosynthetic apparatus of barley plants in the laboratory. Prospects – rhizosphere microorganisms are important components of the environment in the early stages of ontogeny of cereals, so the use of dominant inoculating bacteria has scientific and practical significance in the optimization of agricultural production technologies.

References

Gadzalo Y. M., Patyka N. V., Zarishnyak A. S. (2015). Agrobiologiya rizosfery rasteniy. Monografiya. K.: Agrarna nauka. 386.

Patyka M.V., Tonkha O.L., Sinchenko V.M., Honchar A.M., Patyka T.I. (2019). Osoblyvosti formuvannia strukturovo-funktsionalnoho skladu mikrobiomu chornozemu tsilynnoho v stepu Ukrainy. Mikrobiolohichnyi zhurnal. Vol.81 (№4). P. 90-106.

https://doi.org/10.15407/microbiolj81.04.090

Hadzalo I.M., Patyka M.V., Zaryshniak A.S., Patyka T.I. (2019). Ahromikrobiolohiia z osnovamy biotekhnolohii. Monohrafiia. K.: Ahrarna nauka NAAN. p. 204.

Patyka M.V., Tonkha O.L., Patyka T.I., Kiroiants M.O., Veretiuk S.V. (2018). Otsinka metahenomu prokariotnoho kompleksu chornozemu za ahrarnoho vykorystannia. Mikrobiolohichnyi zhurnal. Vol.80 (№6). P. 109-122. https://doi.org/10.15407/microbiolj80.06.109

Patyka M.V., Kolodiazhnyi O.Y., Ibatullin I.I. (2016). Otsinka metahenomu ta detektsiia funktsionalno znachushchykh polimorfizmiv prokariot hruntu z vykorystanniam metodu pirosekvenuvannia. Mikrobiolohichnyi zhurnal. Vol. 78 (№2). P. 43-51.

https://doi.org/10.15407/microbiolj78.02.043

Lambers H., Mougel C., Jaillard B., Hisinger P. (2009). Plant-microbe interactions in the rhizosphere: an evolutionary perspective. Plant Soil. Vol. 321. - Р. 83-115. https://doi.org/10.1007/s11104-009-0042-x

Paul E. A. (2014). Soil microbiology, ecology and biochemistry. Academic press. 573 р.

Zvyagintseva D. G. (1991). Metodyi pochvennoy mikrobiologii i biohimii. MGU. 304 p.

Downloads

Published

2021-02-25

Issue

No. 1(89) (2021)

Section

Biology, biotechnology, ecology

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).