Technology development of probiotic “Sporo-leks” manufacturing

Authors

  • O. V. Machuskyy National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/dopovidi3(103).2023.013

Keywords:

probiotic, production technology, stability, quality control

Abstract

An important element of the probiotics quality is the technology of their production. There is great variability between the results of preclinical and clinical trials of probiotic microorganisms. To a large extent, this is influenced by the technology and conditions of production of probiotics.

The purpose of the work was to compare the manufacturing technologies of “Sporo-leks” probiotic. Probiotic “Sporo-leks” is a mixture of probiotic cultures of Bacillus licheniformis VK-25 and Bacillus subtilis MK-3, sorbed on a complex activated with the addition of a natural standardized sorbent. Two versions of the “Sporo-leks” probiotic manufacturing technologies were developed: in a liquid nutrient medium and on a dense nutrient medium. During the tests, intermediate quality controls were developed.

As a result of the conducted research, lists of intermediate quality controls were formed during the production of the probiotic “Sporo-leks” in a liquid nutrient medium and on a dense nutrient medium. Dense nutrient media manufacturing has been found to be faster and more cost-effective as it requires only 4 intermediate control stages compared to 5 stages in liquid nutrient media manufacturing. Depending on the stage and production technology, various intermediate studies were carried out, which included microscopy, determination of typicality of growth, determination of contamination by foreign bacterial and fungal microflora, determination of sporulation, determination of the concentration of colony-forming units. Also, during the research, a list of quality control indicators of the finished probiotic “Sporo-leks” was formed and tested. It has been proven that the process of sporulation during the production of the probiotic “Sporo-leks” on a dense nutrient medium occurred faster by 24 hours compared to the technology of production in a liquid nutrient medium. Probiotic production technology on a dense nutrient medium ensured the formation of a more mature spore, which led to the stability of the drug during storage according to the indicator "the number of living cells in 1 g of product." According to the rest of the quality indicators, both technologies equally ensured the stability of the product during storage for 36 months.

References

Riviere J. E., Boothe D. M., Czarnecki-Maulden G. L., Dzanis D. A., Harris P. A., Hendriks W. H., Kirk W. H., Warren L. K. Safety of Dietary Supplements for Hourses, Dogs, and Cats. Washington DC:The National Academies Press; 2009. 513.

Cho S. S., Finocchiaro E. T. Handbook of Prebiotics and Prebiotics Ingredients. Health Benefits and Food Applications. Boca Raton: CRC Press; 2010. 455.

Salem A. F. Z. M. Nutritional Strategies of Animal Feed Additives. New York: Nova Science Publishers; 2013. 231.

Gupta R. C., Srivastava A., Lall R. Nutraceuticals in Veterinary Medicine. Cham, Switzerland: Springer; 2019. 852.

Nivoliez A., Camares O., Paquet-Gachinat M., Bornes S., Forestier C., Veisseire P. Influence of manufacturing processes on in vitro properties of the probiotic strain Lactobacillus rhamnosus Lcr35. Journal of Biotechnology. 2012: 160: 236-241.

Mortazavian A. M., Khorshidian N., Gomes da Cruz A. In Vitro Functionality of Probiotics in Foods. New York: Nova Science Publishers; 2022. 390.

Grzeskowiak Ł., Endo A., Collado M.C., Pelliniemi L.J., Beasley S., Salminen S. The effect of growth media and physical treatments on the adhesion properties of canine probiotics. Journal of Applied Microbiology. 2013: 115: 539-545.

Gioia D. D., Biavati B., Probiotics and Prebiotics in Animal Health and Food Safety. Cham, Switzerland: Springer; 2018. 275.

Drumet L., Tromp Y., Stiegelbauer V. The Development of High-Quality Multispecies Probiotic Formulations: From Bench to Market. Nutrients. 2020: 12: 1-19.

Charalampopoulos D., Rastall R. A. Prebiotics and Probiotics Science and Technology. New York: Springer; 2009. 1273.

Sola-Oladokun B., Culligan E.P., Sleator R.D. Engineered Probiotics: Applications and Biological Containment. Annu. Rev. Food Sci. Technol. 2017; 8:16.1–16.18.

Downloads

Published

2023-06-30

Issue

No. 3/103 (2023)

Section

Veterinary medicine, quality and safety of livestock products

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).