Mineral composition of bees and bee products underfeeding with cerium dioxide
DOI:
https://doi.org/10.31548/dopovidi.1(107).2024.019Keywords:
honeybee, cerium nano-compounds, magnesium, zinc, seleniumAbstract
The search for effective means to prevent diseases of honey bees is a relevant task today, as Ukraine is among the top five producers and exporters of honey to the international market. The use of nano preparations for preventive purposes against viral diseases of bees, as well as for enriching bee products with biologically active compounds, is becoming increasingly widespread. One such means is cerium nano-compounds, particularly its dioxide, which possesses unique antioxidant properties beneficial for both bees and consumers of bee products. The research aimed to investigate the effect of feeding bee colonies with honey containing cerium dioxide on the quality of honey, mineral composition of bee bodies, honey, and beeswax.
The research was conducted in 2023 at the National Scientific Center "Institute of Beekeeping named after P.I. Prokopovich." For the study, two groups of bee colonies of medium strength were formed by the method of analog groups: control and experimental. Spring feeding of bee colonies was carried out with honey containing cerium dioxide at a dose of 1 mm once a day for 14 days, calculated at 1 kg per colony. Control bee colonies were fed natural honey. The quality indicators of honey were determined according to current regulatory documents, and the content of chemical elements (Ce, Mg, Zn, Se) in bee biological substrates, honey, and beeswax was determined by optical emission spectroscopy with inductively coupled plasma on the "Ortima 2100 DV" device (USA).
Feeding bee colonies with honey containing cerium dioxide significantly did not affect the moisture content, proline content in bee honey, and diastase activity of honey. At the same time, an increase in cerium content in bee bodies by 6.2 times (p≤0.05) was found, against a decrease in magnesium content by 13.8% (p≤0.05) and selenium by 14.5% (p≤0.05) compared to the control group. The zinc content in bee bodies under the influence of cerium dioxide did not change. The ability of cerium to accumulate in bee honey under the feeding of bee colonies with honey containing cerium dioxide was proven. At the same time, an increase in cerium content in bee honey by 8.6 times (p≤0.05) against a stable level of magnesium, zinc, and selenium compared to the control group was found.
The use of honey containing cerium dioxide for feeding bee colonies practically did not affect the magnesium, zinc, and selenium content in beeswax but contributed to an increase in cerium content in beeswax by 1.9 times (p≤0.05) compared to the control group. A strong degree of dependence of cerium content in beeswax on its content in bee bodies was found, with r=0.77. The reliability of the approximation is R2 =0.78, that is, 78% of the research data describe this dependence.
References
Andrusyshina, I.M., Lampeka, O.G., Golub, I.O., Lubyanova, I.P., & Kharchenko, T.D. (2014). Methodological recommendations (111) 72.14/133.14 Assessment of mineral metabolism disorders in professional contingents using the method of atomic emission spectrometry with inductively coupled plasma. Avicenna, 60.
DSTU 4497:2005 Natural honeys. Specifications. [Effective from December 28, 2005]. Derzhspozhivstandard of Ukraine, 2005. 21 (National Standards of Ukraine)
Abdi, K., Said, M., & Cherif, A. (2023). The promise of probiotics in honeybee health and disease management. Archives of Microbiology. 205, 73. doi:10.1007/s00203-023-03416-z
Abou-Shaara, H, F., Staron, M., & Staroňová, D. (2020). Potential applications of nanotechnology in apiculture. Entomology and Applied Science Letters, 7 (4), 1-8.
Altunatmaz, S. S., Tarhan, D., Aksu, F., Ozsobaci, N. P., Or, M. E., & Barutçu, U. B. (2018). Levels of chromium, copper, iron, magnesium, manganese, selenium, zinc, cadmium, lead and aluminium of honey varieties produced in Turkey. Food Science and Technology, 39, 392–397. doi:10.1590/fst.19718
Gajger, I. T., Kosanović, M., Oreščanin, V., Kos, S., & Bilandžić, N. (2019). Mineral content in honeybee wax combs as a measurement of the impact of environmental factors. Bulletin of Environmental Contamination and Toxicology, 103(5), 697–703. doi:10.1007/s00128-019-02713-y
Hassona, N.M., & El-Wahed, A.A.A. (2023). Heavy metal concentrations of beeswax (Apis mellifer L.) at different ages. Bulletin of Environmental Contamination and Toxicology, 111, 26. doi:10.1007/s00128-023-03779-5
Kacaniova, M., Knazovicka, V., Melich, M., Fikselova, M., Massanyi, P., Stawarz, R., Hascik, P., Pechociak, T., Kuczkowska, A., & Putała, A. (2009). Environmental concentration of selected elements and relation to physicochemical parameters in honey. Journal of Environmental Science and Health. Part A, Toxic/hazardous substances & environmental engineering, 44(4), 414–422. doi:10.1080/10934520802659802
Kılıç Altun, S., Dinç, H., Paksoy, N., Temamoğulları, F. K., & Savrunlu, M. (2017). Analyses of mineral content and heavy metal of honey samples from south and east region of Turkey by using ICP-MS. International Journal of Analytical Chemistry, 2017, 6391454. doi:10.1155/2017/6391454
Kobyliak, N., Virchenko, O., Falalyeyeva, T., Kondro, M., Beregova, T., Bodnar, P., Shcherbakov, O., Bubnov, R., Caprnda, M., Delev, D., Sabo, J., Kruzliak, P., Rodrigo, L., Opatrilova, R., & Spivak, M. (2017). Cerium dioxide nanoparticles possess anti-inflammatory properties in the conditions of the obesity-associated NAFLD in rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 90, 608–614. doi:10.1016/j.biopha.2017.03.099
Kołacz, R., Iakubchak, O., Taran, T., & Hryb, J. (2023). Safety and quality indicators of rapeseed and sunflower honey from different regions of Ukraine. Ukrainian Journal of Veterinary Sciences, 14(1), 39-56. doi:10.31548/veterinary1.2023.39
Kovalchuk, I., Dvylyuk, I., Lecyk, Y., Dvylyuk, I., & Gutyj, B. (2019). Physiological relationship between content of certain microelements in the tissues of different anatomic sections of the organism of honey bees exposed to citrates of argentum and cuprum. Regulatory Mechanisms in Biosystems, 10(2), 177–181. doi:10.15421/021926
Schulz, M., Łos, A., Grzybek, M., Scibior, R., & Strachecka, A. (2019). Piperine as a new natural supplement with beneficial effects on the life-span and defence system of honeybees. Journal of Agricultural Science, 157, 140–149. doi:10.1017/S0021859619000431
Shcherbakov, A. B., Zholobak, N. M., & Ivanov, V. K. (2020). Biological, biomedical and pharmaceutical applications of cerium oxide. Cerium Oxide (CeO2): Synthesis, Properties and Applications, 279–358. doi:10.1016/b978-0-12-815661-2.00008-6
Tafere, D. A. (2021). Chemical composition and uses of honey: a review. Journal of Food Science and Nutrition Research, 3(4), 194-201 doi:10.26502/jfsnr.2642-11000072
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