Study of the potential for modifying the design of a turbine stirrer blade to reduce the effect of shear stresses on microorganisms in cultivation processes
DOI:
https://doi.org/10.31548/dopovidi.2(108).2024.004Keywords:
bioreactor, stirring, turbine stirrer, shear stress, computer modeling, ANSYSAbstract
Stirring in bioreactors is an important element for the efficient cultivation of cell cultures in biotechnological production. But high-speed stirrers can create high level of shear stresses that negatively affect microorganisms. Therefore, it is important to develop the design of new stirring devices to minimize the negative impact of shear stresses on cells during cultivation. The purpose of this study is to analyze the effect of the designs of turbine stirring devices, proposed by authors, on the parameters of the stirring process during the cultivation of cell cultures by methods of computer modeling. The computer modeling was performed in ANSYS for the process of liquid stirring in a bioreactor. Two new designs of turbine stirrers have been proposed. The idea of the new design is to divide the working blade into two, i.e. to create a cutout in the blade. In the first case, the cutout is a rectangle, in the second - a parallelogram. To compare the efficiency of the proposed designs, we also modeled the stirring with a classical turbine 6-blade stirrer. Based on the modeling results, were obtained contours of the velocity distribution, turbulent kinetic energy, shear strain rate, velocity vectors, and ISO-surfaces forming the core of the rotation vortex. It was found that the presence of cutouts in the turbine stirrer does not lead to a decrease in the velocity of the main flows and redistribution of motion vectors, but significantly reduces the value of turbulent kinetic energy from and shear strain rate. The maximum value of turbulent kinetic energy for the classical stirrer is 2.489 m2/s2, while for the stirrers with cutouts it barely reaches 1.245 m2/s2. The shear stresses decrease by 10 % from 19.63·10-3 Pa for the classical design to 17.67·10-3 Pa for the stirrer with parallelogram-shaped cutouts. The further development of this study will be to analyze the influence of the geometric parameters of the stirrer with parallelogram-shaped cutouts on the qualitative indicators of stirring. The results obtained in this work can be used by engineers and technologists to design bioreactors with reduced values of shear stresses.References
Brandy Sargent. (2017). Managing Shear Stress in Biomanufacturing with the shear protectant Poloxamer 188 – A Discussion. https://cellculturedish.com/managing-shear-stress-in-biomanufacturing-with-the-shear-protectant-poloxamer-188-a-discussion/
Espina, J. A., Cordeiro, M. H., Milivojevic, M., Pajić-Lijaković, I., & Barriga, E. H. (2023). Response of cells and tissues to shear stress. Journal of Cell Science, 136(18), jcs260985. https://doi.org/10.1242/jcs.260985
Huang, K., Tian, Y., Salvi, D., Karwe, M., & Nitin, N. (2018). Influence of Exposure Time, Shear Stress, and Surfactants on Detachment of Escherichia coli O157:H7 from Fresh Lettuce Leaf Surfaces During Washing Process. Food and Bioprocess Technology, 11(3), 621–633. https://doi.org/10.1007/s11947-017-2038-5
Korobiichuk, I., Mel’nick, V., Shybetskyi, V., Kostyk, S., & Kalinina, M. (2022). Optimization of Heat Exchange Plate Geometry by Modeling Physical Processes Using CAD. Energies, 15(4), 1430. https://doi.org/10.3390/en15041430
Korobiichuk, I., Shybetska, N., Shybetskyi, V., & Kostyk, S. (2021). Modeling of Systems of Automated Auxiliary Processes in Pharmaceutical Industry. In R. Szewczyk, C. Zieliński, & M. Kaliczyńska (Eds.), Automation 2021: Recent Achievements in Automation, Robotics and Measurement Techniques (Vol. 1390, pp. 128–135). Springer International Publishing. https://doi.org/10.1007/978-3-030-74893-7_13
Korobiichuk, I., Shybetskyi, V., Kostyk, S., Kalinina, M., & Tsytsiura, A. (2022). Ways to Reduce the Creation of Vortex During Homogenization of Liquid Products. In R. Szewczyk, C. Zieliński, & M. Kaliczyńska (Eds.), Automation 2022: New Solutions and Technologies for Automation, Robotics and Measurement Techniques (Vol. 1427, pp. 329–343). Springer International Publishing. https://doi.org/10.1007/978-3-031-03502-9_33
Maiorano, A. E., Da Silva, E. S., Perna, R. F., Ottoni, C. A., Piccoli, R. A. M., Fernandez, R. C., Maresma, B. G., & De Andrade Rodrigues, M. F. (2020). Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor. Biotechnology Letters, 42(12), 2619–2629. https://doi.org/10.1007/s10529-020-03006-9
Qu, Z., & Breuer, K. S. (2020). Effects of shear-thinning viscosity and viscoelastic stresses on flagellated bacteria motility. Physical Review Fluids, 5(7), 073103. https://doi.org/10.1103/PhysRevFluids.5.073103
Silvani, G., Romanov, V., Cox, C. D., & Martinac, B. (2021). Biomechanical Characterization of Endothelial Cells Exposed to Shear Stress Using Acoustic Force Spectroscopy. Frontiers in Bioengineering and Biotechnology, 9, 612151. https://doi.org/10.3389/fbioe.2021.612151
Singh, V. (1999). Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnology, 30(1/3), 149–158. https://doi.org/10.1023/A:1008025016272
Yang, J., Cheng, S., Li, C., Sun, Y., & Huang, H. (2019). Shear Stress Affects Biofilm Structure and Consequently Current Generation of Bioanode in Microbial Electrochemical Systems (MESs). Frontiers in Microbiology, 10, 398. https://doi.org/10.3389/fmicb.2019.00398
Downloads
Published
Issue
Section
License
Relationship between right holders and users shall be governed by the terms of the license Creative Commons Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).