Pool of free amino acids in the blood plasma of newborn calves under the experimental acidosis
DOI:
https://doi.org/10.31548/dopovidi2016.02.016Keywords:
free amino acids, urea, ammonia, blood plasma, experimental acidosis, newborn calvesAbstract
It was found that during the emergence of calves that are 36 hours old from the state of natural respiratory-metabolic acidosis a gradual increase of concentrations of most of free amino acids in blood plasma, stabilization of the ammonia level and the decrease of the urea content are observed. The peculiarity of the amino acid pool of blood plasma of calves that have never been fed with the colostrum yet is high level of alanine and glycine that subsequently decreases and may indicate the increase of the gluconeogenesis intensity in the liver. Probably, the decrease of the urea concentration and increase of the ammonia content in the blood of intact calves that are 36 hours old is caused by the activation of anabolic processes in tissues in the conditions of the normalization of the blood acid-base parameters and by the beginning of the functioning of kidneys.
Disorders of the acid-base status (ABS) in the form of uncompensated acidosis are extremely widespread in newborn calves. The artificial simulation of the metabolic acidosis state during the first 36 hours of life in calves expresses in a significant increase of the pool of most of free amino acids, especially ketogenic amino acids, with the increase of the urea and ammonia level in the blood. The last may indicate the increase of the intensity of the deamination reactions of amino acids in tissues and decrease of the synthetic abilities of the organism. These changes more and more aggravate the state of acidosis.
Thus, the increase of the Н+concentration in the internal environment of the organism causes changes in the amino acid pool of blood plasma in the newborn ruminants. It certainly will effect on the intensity of the endogenous synthesis of proteins, including the autosynthesis of antibodies and immunity formation.
References
Zakharenko M. A. (1992). Fond svobodnykh aminokislot v tkanyakh telyat v rannem postnatalnom ontogeneze v svyazi s narusheniem funktsionalnogo sostoyaniya zheludochno-kishechnogo trakta [Foundation of free amino acids in the tissues of calves in early postnatal ontogenesis in connection with a violation of the functional state of the gastrointestinal tract]. Agricultural Biology. A series of animal biology, 4, 44-49.
Nishchemenko M. P., Trokoz V. O., Karpovskyi V. I. (2015). Fiziolohichni aspekty vykorystannia aminokyslot dlia pidvyshchennia produktyvnosti tvaryn: monohrafiia [hysiological aspects of amino acids to improve the productivity of animals]. Kyiv: DDP «Ekspo-druk», 253.
Honcharuk (Hryshchenko) V. A. (1998). Osoblyvosti bilkovoho spektra krovi novonarodzhenykh teliat v umovakh zminy parametriv kyslotno-luzhnoho stanu [Features protein spectrum of blood of newborn calves in a changing parameters of acid-base balance]. K, 17.
Melnychuk D. O., Hryshchenko V. A., Tsvilikhovskyi M. I. (2001). Kyslotno-osnovnyi homeostaz orhanizmu novonarodzhenykh teliat [Acid-base homeostasis of the organism newborn calves]. Ukrainian Biochemical Journal, Vol. 73, 6, 81-84.
Melnychuk D. O., Hryshchenko V. A. (2015). Rol kyslotno-luzhnoho stanu ta fosfolipidiv moloka u formuvanni kolostralnoho imunitetu v novonarodzhenykh teliat: monohrafiia [The role of acid-base status and milk phospholipids in the formation kolostralnoho immunity in newborn calves]. Kyiv: TsP «Komprynt», 250.
Melnychuk D. O., Hryshchenko V. A. (2015). Rol molozyva u formuvanni imunitetu v novonarodzhenykh teliat [The role in the formation of colostrum immunity in newborn calves]. Scientific Journal of NULES of Ukraine, 205, 328-335.
Melnychuk D. O., Hryshchenko V. A. (2015). Prohnozuvannia imunodefitsytu v novonarodzhenykh teliat [Prediction immunodeficiency in newborn calves]. Reports of NAS of Ukraine, 11, 107-112.
Melnychuk D. O., Tsvilikhovskyi M. I., Hryshchenko V. A. The method of modeling of metabolic acidosis in newborn calves. Patent of Ukraine for useful model № 2002108184; declared 15.10.2002; published 17.01.2005.
Silakova A. I., Kornyushenko N. P. (1969). Ammiak i glutamin krovi i metody ikh opredeleniya [Ammonia and blood glutamine, and methods for their determination]. Laboratory business, 1, 61-66.
Ovchinnikov Yu. A. (1974). Novye metody analiza aminokislot, peptidov i belkov [New methods of analysis of amino acids, peptides and proteins]. Moscow: Mir, 462.
Kucherenko M. Ye., Babeniuk Yu. D., Voitsitskyi V. M. (2001). Suchasni metody biokhimichnykh doslidzhen [Modern methods of biochemical research]. Kyiv: Fitosotsiotsentr, 424.
Melnichuk D. A. (1989). Metabolicheskaya sistema kislotno-shchelochnogo gomeostaza v organizme cheloveka i zhivotnykh [The metabolic system of acid-base homeostasis in humans and animals]. Ukrainian Biochemical Journal, Vol. 61, 3, 3-21.
Downloads
Issue
Section
License
Relationship between right holders and users shall be governed by the terms of the license Creative Commons Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
