BLOOD INDICATORS common carp WHEN USING probiotic microorganisms (BACILLUS SUBTILIS And LACTOBACILLUS ACIDOPHILUS) IN COMPLEX AND monoculture

Abstract

Due to the restriction on the use of antibiotics in the fisheries sector for the prevention and elimination of outbreaks fish became increasingly important to biotic drugs. They can not only optimize the balance of normal microflora of fish which regulates physiological functions, but act as antagonists of pathogens without much damage as a result of treatments. One tool control of the body of fish for the use of probiotics is to study quantitative and qualitative composition of white blood cells carp. Indirect marker changes in the immune system of fish is the level of ALT and AST, protein ratio and the level of albumin and globulins. Blood is a sensitive and informative indicator of body carp, which quickly responds to the impact of exogenous and endogenous factors on individual species and the population of fish in general. In peripheral blood of this species of fish are young and mature forms of red blood cells, white blood cells and platelets

Blood is a sensitive and informative indicator of the condition of the body, quick to respond to the impact of exogenous and endogenous factors on the individual and the individual in the population of fish in general. The carp in peripheral blood are young and mature forms of red cells, white cells and platelets. Special morphological differences in the maturity stages of red blood cells and white blood cells in the carp were observed. In the blood of carp are present all groups of granulocytes: neutrophils, basophils, eosinophils, and basophils pseudo. The most powerful in determining the immune status of the fish indicators of quantity and ratio of different groups of white blood cells. The aim of studies compare the effect of white on the body of common carp probiotic microorganisms Bacillus subtilis and Lactobacillus acidophilus in the complex and use of monocultures. 40 carp specimens were used to study ordinary. The fish was brought to Trigorskoe reservoir and a number of private brood ponds. When processing the data obtained after the studies have shown that the use of the above complex of probiotic microorganisms is more effective than the use of monocultures. It can be concluded about the possibility of using the recommendations of the complex or analogs for growing fish pond.

Keywords: carp, a complex of probiotic microorganisms, Bacillus subtilis, Lactobacillus acidophilus, blood, erythrocytes, leukocytes, differential white blood cell count.

At the end of the experiment (1 month) were determined and compared the degree of influence of probiotic microorganisms in the body carp.

Period feeding feed with probiotic microorganisms lasted from late February to early March. Results of changes in the blood due to the use of probiotic microorganisms are listed in the tables.

The level of hemoglobin after use of biotic sector increased by 31.8%, the number of red blood cells increased by 12.5%. When using Bacillus subtilis monoculture hemoglobin and red blood cells increased by 8% and 7.5% respectively. Monoculture Lactobacillus acidophilus using induced no significant changes.

The total number of white blood cells before the experiment was 4.5 t / L. After application of complex probiotic microorganisms that number increased to 7,0 T / L, using Bacillus subtilis monoculture leukocyte count was 5,4 T / L. The use of monoculture Lactobacillus acidophilus has not led to significant changes and the number of white blood cells was 4,9 T / L. That data using complex cultures of microorganisms to 29.65% more efficient than using Bacillus subtilis as a monoculture. Bacillus subtilis in monoculture currently the most common probiotic use in animal husbandry and fisheries.

Was seen from the above data leukocyte formula shift to the right was active in the application of complex Bacillus subtilis and Lactobacillus acidophilus, but less intensive monoculture using Bacillus subtilis (7%). The use of Lactobacillus acidophilus as an independent culture to bring significant changes.

Data show significantly higher functional activity systems T-lymphocytes in the blood of carp grown in applying experimental probiotic. The number of B-lymphocytes in the blood decreased by 9%.

Also, the feeding of probiotic complex there is increasing serum total protein (21.1%) and the interest adjustment amount of albumin and globulins. Index values for total protein complex was the best application for the use of monocultures by 17%. In the study of enzymes AST noticeable decrease in the number and value alignment AST and ALT.

Conclusions can be said as a pilot Application of probiotic based on Bacillus subtilis and Lactobacillus acidophilus gave more positive results in qualitative and quantitative changes in blood parameters sohorichok carp than the use of these crops separately. It turned out in increasing increase of hemoglobin (complex gave the best performance at 20%) and red blood cells in the red blood (5%), offset leykoformuly right has also been a noticeable (~ 7%) using two microorganisms simultaneously, and gave a more positive trend in increasing the number of white blood cells.

Based on these studies can be argued that the use of probiotic complex from Bacillus subtilis and Lactobacillus acidophilus can be recommended for use in breeding farms of all types as a promising alternative to antibiotics and many means instead of synthetic immunomodulatory drugs, as well as replacement of the application monocultures with feeding fish.