PATHOLOGICAL CHANGES IN WHITE MICE ORGANISM UNDER THE INFLUENCE OF VARIOUS DOSES OF DISINFECTANT "UNIVAYT"

Abstract

Condition of the body at the cellular level is one of the most informative indicators on the impact of these substances directly on the organism. The influence of the substance on the cells can be explored through the histological examination.

Analysis of recent researches and publications. We have previously studied methods of acute toxicity disinfectant "Univayt" on biological objects. However, a number of scientists who have studied the action of disinfectants argue that factors such as the clinical condition of the animals, hematological, toxicological data, but also to assess the impact of toxic substances on humans and animals, but did not allow to analyze the action directly on the cell.

The aim was to conduct pathological and anatomical and histological examination of internal organs of laboratory animals, after the exposure on them of the disinfectant "Univayt."

Materials and methods. The study examined was the impact on animals organism of the new disinfectant "Univayt", which was developed based on organic acids and nanoparticles of Argentum and Cuprum. For research needs was formed control and two research groups of white mice, 10 species in each group. Mice of the first experimental group was administered orally substance in a 0.5% concentration dose of 1 cm³, the second - lethal concentration of disinfectant. For experiments were selected clinically healthy white mice weighing 18 – 20 g.

To study the effect of working and lethal concentrations of disinfectant "Univayt" on the body of laboratory animals was performed euthanasia of the white mice, using ether anesthesia, which is consistent with the Regulation "On protection of animals from cruelty" and Regulations "Of the European Convention for the Protection of vertebrate animals used for experimental and other scientific purposes" (Strasbourg, 1985).

Corpses of animals after euthanasia were cutted, conducted their macroscopic research, organs were taken for histological examination, namely, heart, lungs, kidneys, liver, spleen. The samples were fixed in 10% aqueous solution of formalin, embedded in tseloyidyn, made cuts of 15 microns thick, stained with hematoxylin and eosin Karatsu, examined under a light microscope, micrographs were produced.

Results and discussion. Macroscopic changes. During the autopsy of mice as a control and 1 experimental group showed no macroscopic changes. This indicates the low toxicity and harmless disinfectant when it gets to working concentrations in the body.

During the autopsy the mice corpses’ of the 2 research group were observed edema and congestive lung congestion, dilatation of the right atrium and ventricle, blood overflow spleen, bleeding and vascular congestion in the liver.

Microscopic changes. During the microscopic examination, it was found that in the tissue samples from animals of the 1 experimental group were no pathological changes that are discovered, their microscopic structure was identical to that of animals in the control group.

The natural structure of the mice myocardium as 1 experimental and control groups were similar. Cardiomyocytes are homogeneous and uniform coloration of the cytoplasm. Basophilic nuclei of cells, elongated shape. Occasionally there are isolated cardiomyocytes with increased enlightened nuclei elongated-oval. The cytoplasm of cells in slightly enlightened.

In carrying out histological study of the lungs was found no change in the mice to which were administrated a 0.5% working solution "Univayt."

In renal glomerulus vascular changes were detected. The epithelium of the renal tubules cubic form with clear contours. Kidney interstitium without visible changes, with moderately filled vessels. Histostructure significant differences in the kidneys of mice control and 1 experimental group were found.

The structure is completely preserved liver tissue, hepatocytes are irregular polygonal shape, with a pronounced eosinophilic grain, with no signs of degeneration or necrosis. Bile capillaries is not extended.

By histological structure of the liver of animals one experimental group did not differ from controls.

Spleen animals of the first experimental group with the normal ratio of red and white pulp. Sinusoidal vessels moderately filled with blood red pulp. Among the red pulp cells are isolated macrophages, which cytoplasm is filled with brown pigment gemosiderin. White pulp presented lymphoid nodules, which are small size light centers. All the parenchyma of the spleen is riddled trabeculae arranged in different directions. Histostructure of the spleen of animals of the first research group is similar to that of animals of the control group mice.

Investigation of the lethal dose of disinfectant accompanied by death of white mice.

During the microscopic examination of tissue samples taken from the animals two research groups discovered these lesions. In the lung alveoli are filled with homogeneous content weak eosinophilic content, vessels dilated, full of blood. The same content found in the bronchioles. In the myocardium individual cells - cardiomyocytes were destroyed. In the kidney glomeruli observed in vascular mesangiocytes destruction, the increase in size of the glomeruli, the accumulation of infiltration in the space between vessels and glomerular capsule. The vessels of the spleen were enlarged and full of blood. In the cytoplasm of liver hepatocytes was not uniform, foamy appearance, their nuclei were poorly stained vessels (as internallobular and interlobular) extended and full of blood.

Conclusions

1. Our studies on the impact of the new disinfectant "Univayt" at 0.5% concentration in the body of white mice in his oral use in volume 1 cm³, showed that any macroscopic and microscopic changes in the organs and tissues of animals first research group were found.

2. Lethal doses agent causing pathological changes in organs and tissues that are characteristic of intoxication external origin. Revealed changes suggest the development of pathological processes such as acute venous congestion of internal organs, granular dystrophy of hepatocytes and epithelial renal tubular necrosis cardiomyocytes, stromal hemorrhages in internal organs hyperemia and edema of the lungs (which is the direct cause of death of animals of the second experimental group).