PROLIFERATIVE ACTIVITY OF RAT MESENCHYMAL STEM CELL AT INFLUENCE CULTURE MEDIUM
DOI:
https://doi.org/10.31548/dopovidi2016.03.018Keywords:
mesenchymal stem cells, proliferation, culture medium, differentiationAbstract
Mesenchymal stem cells (MSCs) are bone marrow populating cells, different from hematopoietic stem cells, which possess an extensive proliferative potential and ability to differentiate into various cell types, including: osteocytes, adipocytes, chondrocytes, myocytes, cardiomyocytes and neurons. MSCs play a key role in the maintenance of bone marrow homeostasis and regulate the maturation of both hematopoietic and non-hematopoietic cells. With published data we are known about the effectiveness of using stem cells in degenerative processes in the organs.
Therefore, we can argue about expediency of use cell technologies for the treatment degenerative pathology’s. MSCs seem to represent a future powerful tool in regenerative medicine, therefore their research are particularly important in clinical research.
Goal of the work. Investigate the proliferative activity of MSCs depending on the type used by culture media and to establish the ability of mesenchymal stem cells to differentiate into kardiomyogenic direction under the influence of exogenous factors.
Materials and methods. In studies using MSCs, obtained from 12-day not linear rats. We compared the impact of three different culture media on proliferative activity derived cells. We investigated the ability of MSCs to differentiate into kardiomyogenic direction under the influence of 5 - azacytidine.
Results of research and discussion. Was investigated influence of three different composition culture medium on proliferative activity of MSCs. Was defined the better culture medium. Proved that mesenchymal stem cells are able of aimed differentiation at kardiomyogenic under the direction of transcription factor 5 azacytidine. The data confirmed the morphological assessment of culture and research at the molecular level.
Conclusions.
- Cultivation the rat mesenchymal stem cells in DMEM F12 - D8437 and ECT - 20% with the addition of antibiotic-antimycotics 10 ml / cm3 provide the highest proliferative activity.
- Mesenchymal stem cells are capable of aimed cardiomyogenic differentiation under the direction of transcription factor 5 azacytidine.
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