DEVELOPMENT OF A NEW METHOD OF DIAGNOSTICS OF TOXOPLASMOSIS BASED ON PHOTOLUMINESCENCE OF ZINC OXIDE NANOPARTICLES
DOI:
https://doi.org/10.31548/dopovidi2016.07.011Keywords:
toxoplasmosis, Toxoplasma gondii, diagnostics, optical biosensor, zinc oxide, photoluminescenceAbstract
Toxoplasma gondii is a common agent of protozoan disease of different kinds of animals and humans. Today it is registered in Europe, Asia, Africa, North and Latin America. That is why timely and accurate diagnosis of the disease is important. To the commonly used diagnostic methods for toxoplasmosis belong complement fixation, prolonged reaction of complement fixation, fluorescent antibody reaction, the reaction of indirect immunofluorescence, agglutination test, indirect hemagglutination reaction, the reaction latexagglutination, enzyme linked immunosorbent method, widely used polymerase chain reaction. However, these methods are long-term, expensive, difficult, requiring high professional training of specialists and special equipment and reagents. That is why a new method for the diagnosis of toxoplasmosis based on photoluminescence of nanoparticles of zinc oxide was developed and tested. Photoluminescence spectra of Toxoplasma biomolecules on the surface of ZnO was recorded through fiber-optic spectrometer, and the method of scanning electron microscopy was used to study the nanorods microstructure.
ZnO nanostructures have a number of specific properties that make them attractive for use in bioanalytical measurements and design of biosensors. Due to the unique combination of fluorescent, semiconductor and piezoelectric properties of ZnO can be used to create sensors that are based on different principles registration signal.
As a result of studies it was revealed that immune complexes biosensor based on zinc oxide have sufficient specificity reaction taking into account the significant reduction of signal when making specific serums with the dilution of 1: 5. Reduction of the photoluminescence intensity indicates the formation of nanorods biocomplexes on the surface of zinc oxide on a "key-lock" principle which is accompanied by a modification of the pre-adsorbed antibody molecules structure.
Based on the obtained results, it is possible to state that described method has high detection rate and relatively low complexity of the application. Further study and improvement of methods of diagnosis of toxoplasmosis using immune biosensor will enable cheaper diagnosis of the disease process and accelerate it.
References
Ahn, K.Y., Kwon, K., et al. (2011). A sensitive diagnostic assay of rheumatoid arthritis using three-dimensional ZnO nanorod structure // Biosensors & Bioelectronics, 28 (1), 378-385.
https://doi.org/10.1016/j.bios.2011.07.052
Dubey J. P., Jones J. L. (2008). Toxoplasma gondii infection in humans and animals in the United States. Internal Journal оf Parasitology, 38, 1257−1278.
https://doi.org/10.1016/j.ijpara.2008.03.007
Jiang S., Hua E., Liang M., Liu B., Xie G. (2013) A novel immunosensor for detecting Toxoplasma gondii-specific IgM based on goldmag nanoparticles and graphene sheets. Colloids Surf. B. Biointerfaces, 101, 481−486.
https://doi.org/10.1016/j.colsurfb.2012.07.021
Khranovskyy V, Lazorenko V, Lashkarev G, Yakimova R. (2012) Luminescence anisotropy of ZnOmicrorods. Journal of Luminescence, 132 (10), 2643-2647.
https://doi.org/10.1016/j.jlumin.2012.04.048
Luo Y., Liu X., Jiang T., Liao P., Fu W. (2013) Dual-aptamer-based biosensing of toxoplasma antibody. Anal Chem., 85(17), 8354−8360.
https://doi.org/10.1021/ac401755s
Meng K., Sun W., Zhao P., Zhang L., Cai D., Cheng Z., Guo H., Liu J., Yang D., Wang S., Chai T. (2014) Development of colloidal gold-based immunochromatographic assay for rapid detection of Mycoplasma suis in porcine plasma. BiosensBioelectron, 55, 396−399.
https://doi.org/10.1016/j.bios.2013.12.048
Mihailova, V. Gerbreders, E. Tamanis, E. Sledevskis, R. Viter, P. Sarajevs (2013). Synthesis of ZnOnanoneedles by thermal oxidation of Zn thin films, Journal of Non-Crystalline Solids, 377, 212-216.
https://doi.org/10.1016/j.jnoncrysol.2013.05.003
Starodub N.F. (2013) Biosensors for the Control of Biochemical Parameters in the Diagnostics of Diseases.Book of series in sensors, Portable Biosensing of Food Toxicants and Environmental Pollutants, CRC Press, Taylor&Francis Croup Boca Raton London, New York, 743-775.
https://doi.org/10.1201/b15589-29
Viter R., V. Khranovskyy, N. Starodub, et. al. (2014). Application of Room Temperature Photoluminescence From ZnO Nano-rods for Salmonella Detection, IEEE Sensors Journal, 14(6), 2028-2034.
https://doi.org/10.1109/JSEN.2014.2309277
Wang H., Lei C., Li J., Wu Z., Shen G., Yu R. (2014) A piezoelectric immunoagglutination assay for Toxoplasma gondii antibodies using gold nanoparticles. Biosens Bioelectron, 19(7), 701−709.
https://doi.org/10.1016/S0956-5663(03)00265-3
Yakimova R., Selegard L., et al. (2012) ZnO materials and surface tailoring for biosensing. Frontiers in bioscience (Elite edition), 4, 254-278.
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