THE OPTIMIZATION OF CRYOPROTECTIVE ENVIRONMENT FOR Sperm Cryo preservation in sturgeon(ACIPENSER RUTHENUS, L. 1758)
DOI:
https://doi.org/10.31548/dopovidi2017.02.013Keywords:
sturgeon, cryopreservation, fertilizing, embryonic development, creatine, Carassius gibelio plasmaAbstract
The issue of cryopreservation of sturgeon sperm is lively considered by researchers and scientists. Many scientists study this issue as this species is dramatically decreasing in natural waters. It is possible to solve this problem if to use an artificial reproduction at farms and to stock natural waters with juvenile fish. The problem is the lack of high quality sperm of ripe male sturgeon. One of the ways to tackle this problem is sperm cryopreservation. It is confirmed by many researches. However most of them study how to enhance the process of sperm cryopreservation in order to provide a higher percentage of spermatozoon survival. We need more information about the quality and full value of fish hatched from eggs fertilized with cryopreserved sperm
The aim of the study is to optimize elements of cryoprotectant solution which is used to freeze male sturgeon semen and to study the influence of elements of cryo solution on the quality and vital capacity of juvenile fish which can be used for further reproduction and stocking natural waters with sturgeon.
The experiment was conducted at an educational and research and training laboratory of fishery of the department of aqua culture of NUBIP of Ukraine. The object of the study is male sturgeon, its semen, eggs, juvenile fish hatched from eggs fertilized with native and cryopreserved semen. Traditional in fishery and cryobiology techniques were used during the experiment. The cryopreservation process was evaluated using the parameter of semen fertilizing capacity. The quality of juvenile fish was evaluated by calculating linear and weight parameters of juvenile fish for three months and by comparing testing and experimental groups.
The study resulted in optimization of elements of cryoprotectant environment to freeze sturgeon sperm. Creatine and fructose were used as modificators which improved the quality of thawed sperm and preserved the fertilizing capacity of sperm at the level of a testing group. The use of Carassius gibelio plasma which had undergone natural cold acclimation did not confirm the presupposed result. It can be because of the osmolality of the environment and this phenomenon needs further studying.
The study of eggs shows that there are no significant differences in embryonic development (from fertilizing till the middle of egg dissection and from the end of eggs dissection till early gastrula) between comparative indicators of experimental and testing groups. It confirms the efficient conditions for a fertilizing process and semen incubation.
Eggs and juvenile fish hatched from eggs fertilized by cryopreserved sperm and grown for three months are characterized by better linear and weight parameters in comparison with control
The results show that method of cryopreservation is useful to freeze sturgeon sperm in order to get high quality fish.
The results confirm the hypothesis about the usefulness of cryopreserved sperm to meet the needs of aquaculture. The optimization of elements of cryoprotectant environment and freezing make it possible to preserve spermatozoons. The focus of further study is getting ripe female sturgeon using cryo techniques.
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