OF THE NATURAL DEFENSE MECHANISM IN CARP SCALELESS AND CARP UNDER VITAMINS AND MINERALS
DOI:
https://doi.org/10.31548/dopovidi2017.02.021Keywords:
Carp, fat-soluble vitamins, iodine, zinc, selenium, indicators of natural immunityAbstract
The purpose of the research was to elucidate the impact of fat-soluble vitamins and trace elements selenium, zinc and iodine in the composition of dietary intake to activity of cellular and humoral mechanisms of nonspecific resistance in carp and wild carp.
Experiments conducted on two groups of fish (wild carp and scaleless carp) age of two years, which in principle analogues were divided into two control and two experimental groups of 10 individuals each. Fish kept in special trays under conditions of continuous closed system of water circulation. Fishes of control groups within 30 days were fed with granulated feed. Individual research groups were fed with a similar feed additives drug "Tryvit" in calculating the amount of 2,500 IU of vitamin A, 3333 IU of vitamin D3, vitamin E 1,7 mg and 5 mg / kg of potassium iodide, 40 mg / kg zinc sulphate and 0 3 mg / kg sodium selenite per kg of feed.
The material for the studywas blood a month after the fish feeding investigated mineral-vitamin complex. Serum samples wereinvestigated bactericidal (BASK) and lizocym (LASK) activity and the content of circulating immune complexes (CIC) average molecular weight.
In whole blood was determined phagocyte activity (FA) leukocytes calculated phagocyte number (SF) and phagocyte index (FI). The definition of these indicators generally accepted methods performed in accordance with the recommendations described Mikryakovym R. (1991).
The stimulating effect of fat-soluble vitamins and trace elements selenium, zinc and iodine in the composition of feed additives to the activity of cellular and humoral body's natural resistance scaleles carp and carp has beenestablished. In particular, the fish of both experimental groups compared to the control found higher (p <0,05-0,01) phagocytic and bactericidal activity of blood, and the carp - lizocym activity of serum. It should be noted that the effect of the studied additives was more pronounced in the body carp than scaleles carp.
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