INFLUENCE OF BACTERIOPHAGE DRUGS ON THE PROCESS FILMFORMATION STAPHYLOCOCCUS AUREUS STRAINS

Authors

  • E. S. Vorobey Oles Honchar Dnipropetrovsk National University , Дніпропетровський національний університет імені Олеся Гончара
  • O. S. Voronkova Oles Honchar Dnipropetrovsk National University , Дніпропетровський національний університет імені Олеся Гончара
  • A. I. Vinnikov Oles Honchar Dnipropetrovsk National University , Дніпропетровський національний університет імені Олеся Гончара

DOI:

https://doi.org/10.31548/dopovidi2017.03.007

Keywords:

antibioticresistance, staphylococcus, biofilm, bacteriophages

Abstract

  High level of resistance to antibiotics of bacteria in biofilms causes an actuality of finding of additional preparates for treatment of infections caused by film-forming bacteria. As one of the same preparations are bacteriophages.

Maximal efficacy of inhibition of film growth was during use of piobactariophage polyvalent. Significant changes were fixed during it use on daily biofilm. It was shown that decrease of: amond of CFU in plankton in 115.00 ± 5.44 times, amond of CFU in biofilm – in 63.90 ± 2.10 times, dry weight of biofilms – on 34.47 ± 5.29%, conteine of matrix protein – on 41.77 ± 7.14%, and optical density of biofilms – on 47.27 ± 10.24% compared to control of 2 day biofilm. Minimal efficacy took place during bacteriophage staphylococcal liquid action. It maximal effect took place during addition of this preparate on 2 day biofilm. It was shown a decrease of  planktonic CFU in 83.17 ± 3.55 times, CFU in biofilm in 62.20 ± 3.04 times, dry weight of biofilms – on 32.67 ± 5.75%, contein of matrix protein – on 37.17 ± 3.53%, and optical density of biofilms – on 42.67 ± 7.47% compared to 3 day biofilm.

Given data can make a conclusion the bacteriophages may have great place for medicine and agriculture for reduce of use of antibiotics. 

References

Katter, Eh., Sulakvelidze A. ed. (2012). Bakteriofagi: biologiya i prakticheskoe primenenie [Bacteriophages: Biology and Practical Applications]. Moscow: The scientific world, 640.

Gostev, V. V., Sidorenko V. V. (2010). Bakterial'nye bioplenki i infekcii [Bacterial biofilms and infections]. Journal Infectology, 2 (3), 4-15. doi:http://dx.doi.org/10.22625/2072-6732-2010-2-3-4-15

Lyamin, A. V., Botkin, E. A., Zhestkov, A. V. (2012). Problemy v medicine, svyazannye s bakterial'nymi plenkami [Medical Problems Associated with Bacterial Biofilms]. Clinical microbiology and antimicrobial chemotherapy, 14 (4), 268- 275.

Novahova, Zh. D., Budanov, P. V., Strizhakov, A. N., Churganova, A. A. (2014). Sovremennye vozmozhnosti selektivnoj antibakterial'noj terapii v akusherstve i ginekologii [Modern Opportunities for Selective Antibiotic Treatment in Obstetrics and Gynecology]. Difficult patient, 12 (12), 36-38.

Smirnova, T. A. Didenko, L. V., Azizbekyan, R. R., Romanova, Yu. M. (2010). Strukturno-funkcional'naya harakteristika bakterial'nyh bioplenok [Structural and functional characteristics of bacterial biofilms]. Microbiology, 79 (4), 435-446.

https://doi.org/10.1134/S0026261710040016

Agarwal, A., Singh, K. P., Jain, A. (2010). Medical significance and management of staphylococcal biofilm. FEMS Immunology & Medical Microbiology, 58 (2), 147-160.

https://doi.org/10.1111/j.1574-695X.2009.00601.x

Alves, D. R., Perez Esteban, P., Arnot, T. C., Harper, D. R., Kot, W., Hansen, L. H., Enright, M. C., Jenkins, A. T. (2014). Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm formation. Applied and Environmental Microbiology, 80 (21), 6694-6703.

https://doi.org/10.1128/AEM.01789-14

Bryers, J. D. (2008). Medical biofilms. Biotechnology and Bioengineering, 100 (1), 1-18.

https://doi.org/10.1002/bit.21838

Del Pozo, J. L., Alonso, M., Arciola, C. R., Gonzalez, R., Leiva, J., Lasa, I., Penades, J. (2007). Biotechnological war against biofilms. Could phages mean the end of device-related infections? The International Journal of Artificial Organs, 30 (9), 805-812.

https://doi.org/10.1177/039139880703000910

Garrett, T. R., Bhakoo, M., Zhang, Z. (2008). Bacterial adhesion and biofilms on surfaces. Progress in Natural Science, 18 (9), 1049-1056.

https://doi.org/10.1016/j.pnsc.2008.04.001

Gilbert, P., Allison, D. G., McBain A. J. (2002). Biofilms in vitro and in vivo: do singular mechanisms imply cross-resistance? Journal of Applied Microbiology, 92 (s1), 98S-110S.

https://doi.org/10.1046/j.1365-2672.92.5s1.5.x

Hoiby, N., Bjarnsholt, T., Givskov, M., Molin, S., Ciofu, O. (2010). Antibiotic resistance of bacterial biofilms. International Journal of Antimicrobial Agents, 35, (4), 322-332.

https://doi.org/10.1016/j.ijantimicag.2009.12.011

Lungren, M. P., Christensen, D., Kankotia, R., Falk, I., Paxton, B. E., Kim, C. Y. (2013). Bacteriophage K for reduction of Staphylococcus aureus biofilm on central venous catheter material. Bacteriophage, 3 (4), e26825.

https://doi.org/10.4161/bact.26825

O'Flaherty, S., Ross, R. P., Coffey, A. (2009). Bacteriophage and their lysins for elimination of infectious bacteria. FEMS Microbiology Reviews, 33 (4), 801-819.

https://doi.org/10.1111/j.1574-6976.2009.00176.x

Otto, M. (2008). Staphylococcal biofilms. Current Topics in Microbiology and Immunology, 322, 207-228.

https://doi.org/10.1007/978-3-540-75418-3_10

Römling, U., Balsalobre, C. (2012). Biofilm infections, their resilience to therapy and innovative treatment strategies. Journal of Internal Medicine, 272 (6), 541-561.

https://doi.org/10.1111/joim.12004

Taj, Y., Essa, F., Aziz, F., Kazmi, S. U. (2012). Study on biofilm-forming properties of clinical isolates of Staphylococcus aureus. The Journal of Infection in Developing Countries, 6 (5), 403-409.

https://doi.org/10.3855/jidc.1743

Wu, H., Moser, C., Wang H.-Z., Hoiby N., Song Z.-J. (2015). Strategies for combating bacterial biofilm infections. International Journal of Oral Science, 7 (1), 1-7.

https://doi.org/10.1038/ijos.2014.65

Downloads

Published

2017-06-15

Issue

No. 3(67) (2017)

Section

Biology, biotechnology, ecology

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).