PECULIARITIES OF ARBORIZATION OF THE SECRET OF MISSILE SHELLS IN COWS

Abstract

 It was discovered that process and structure of arborization changes during sexual cycle. Arborization in mucous of eye conjunctiva forms from small interspersing, whereof develop small twisted stems of fern with many branched stalks. We also noted single “snowflake” crystallization. During examining of swabs in the place of the biggest accumulation of liquid occur shiny drop were arborization is formed. We discovered that arborization in eye conjunctiva occurs during the sexual cycle regardless its stage or pregnancy, so studying such material is not informative for diagnosing proestrus and estrus of sexual cycle of cows.

While analyzing nasal mucous we observed thin stems of fern with a lot of endless branching which are develop from drops and form branched crystallization. We observed arborization during sexual cycle regardless its stage. Hereafter during storing of swabs we noticed single fern stems with many interspersing. The decay occurred since 7 till 9 day at +19-22 С^о. If temperature was lower the speed of decay decreased.

Thus after analyzing of eye conjunctiva and nasal swabs we can state that their structure is similar. The stems are thin with multiple branches develop from small interspersing. During storing swab decays with traces of arborization. Considering organoleptic peculiarities of eye and nasal mucous which show constant arborization we discovered that such method is less informative for determination of optimal time of insemination and diagnosing early terms of pregnancy.

During examination of oscular swabs we observed changes in picture during sexual cycle - ample twisted leaves of fern which loosely jointed together and formed branched crystallization (in contrast to eye and nasal swabs). During this stage of estrus at hundredfold magnification we noted skeletonization in the form of fern leaves, clarity of the picture and signified embranchments. We believe that it occurs at the expense of increasing of estrogens and salts NaCI, KCI та KВr. Such arborization was observed both during spontaneous and hormonal stimulated heat.

Hereafter while controlling the process of sexual cycle, in saliva swabs we observed single crystallization in the shape of snowflakes with distinct center with small embranchments. Decreasing the level of estrogen and increasing concentration of progesterone is characteristic for such crystallization. Such picture we usually observed during the ovulation.

It is characteristic for arborization in saliva that image in the shape of fern leave is barely visible. Sometimes there are rods and single crystals which are not fully crystallize. We believe that such vague picture correlates with hyperestrogenization and coincides with metoestrus.

Thus we conclude that upon occurrence of ovulation, saliva crystallization reaches its maximum due to the increasing in estrogens and salts NaCI, KCI та KВr. During metoestrus we observed picture fragmentation on object-plate with formation of single or vague light crystals.

During studying the vaginal mucus swabs we observed different arborization which changed according to the stage of sexual cycle of cows. In this period we always noted crystallization in the shape of fern leaves, but at hundredfold magnification the image was like skeletonization.

Arborization with light and vague image is indicative for ending of estrus and beginning of metoestrus. There were noted a lot of “fern-leave crystals with twisted stems” which occupy all ocular view. Stems are very long, thin with multiple embranchments.  

At metoestrus stage microscopic image on object-plate was like snowflake single fern-leaves. It indicates on such stage of sexual cycle during which progesterone inhibits the influence of estrogens. At this phase arborization forms in one point and from this the snowflake-like image begins. Embranchments are almost always thick and are usually situated on periphery of object-plate or diffusive.

Arborization of mucous from vagina and cavitas oris can be used as additional diagnosing and prognostic test for determination the optimum time for insemination.