Interconnection between the kallikrein-kinin system and endogenous intoxication of rats of different age in the conditions of the early and late polytrauma
DOI:
https://doi.org/10.31548/dopovidi2018.02.009Abstract
There is a clear relationship between age and level of mortality in the injured with a polytrauma. The older the injured are, the higher the mortality. In view of this, the actual task is to examine various aspects of traumatic disease both in humans and animals of all ages at an early and late terms after a polytrauma.
Important pathogenetic mechanisms of traumatic disease are the presence of violations of the kallikrein-kinin system (KKS) and the development of endogenous intoxication (EI). The status of the KKS reflects the activity of the inflammatory reaction in the body, the state of microcirculation, the morbidity.
The detoxification system failure leads to the development of EI syndrome, which accompanies the disease and complications associated with increased tissue disintegration, increased catabolism, insufficient function of internal organs.
Goal of research – establish interrelation between the kallikrein-kinin system and endogenous intoxication in the early and late terms after the polytrauma modeling.
The research was conducted with 156 non-linear white rats at the age of 3, 6 and 24 months by polytrauma modeling. The material for biochemical research was taken at 1, 3, 24 hour and 14 day after injury.
Activation of KKS confirms the presence of inflammatory process in animals with polytrauma. Most noticeable they increase in young animals. After polytrauma modeling of all examined age groups of animals, the increase of specific proteolysis by the KK can indicate the activation of adaptive protective reserves. With age, there is a slight exhaustion of nocifensor, as evidenced by the data we received about a less pronounced increase KK of older rats at the age of 24 months, compared to young and mature animals.
The content of medium-weight molecules (MWM254 and MWM280) increase at an early and late terms after the polytrauma modeling in all age groups of animals compared to intact rats, indicating a pronounced increase both the chain and aromatic amino acids in the peptide components of MWM. In our research, the increase of MWM254 of rat blood in the three age groups was observed in the early stages after the polytrauma modeling, with a maximum value of 24 hour. The growth of the MWM content of the animal organism after the polytrauma modeling indicates an increase in catabolic processes. Increasing the content of MWM254, which may include oligopeptides, nucleic acid fragments, higher fatty acids, triglycerides, may indicate the hepatocyte membranes failure, and MSM280, components of which may be purine bases, uric acid and aromatic amino acids - an inhibition of detoxification function of the liver.
Activation of the examined parameters confirms the presence of inflammatory process in animals with polytrauma, and there is a tendency to its greater increase in young animals. Data were obtained in favor of stable activation of total proteolysis in mature and elderly animals.
A promising direction of further research is the possibility of using an experimental model for screening study of the further course of the traumatic process, taking into account the age and period of traumatic injury, changes in the kallikrein-kinin system and the degree of rise of intoxication syndrome.
References
Campbell, D. J. (2001). The Kallikrein-Kinin System In Humans. Clinical and Experimental Pharmacology and Psychology, 28 (12), 1060-1065.
https://doi.org/10.1046/j.1440-1681.2001.03564.x
Farmer, S. G. (1997). The Kinin System. New-York: Academic Press, 349.
Moreau, M. E., Garbacki, N., Molinaro, G., Brown, N. J., Marceau, F, & Adam, A. (2005). The Kallikrein-Kinin System: Current and Future Pharmacological Targets. Journal of Pharmacological Sciences., 99, 6-38.
https://doi.org/10.1254/jphs.SRJ05001X
Margolen, V., Sommerhoff, Ch. P., Fritz, H., & Schmitt, M., ed. (2013). Kallikrein-relaterd peptidases: Characterization, regulation, and interactions within the protease web. Berlin: Walter de Gruyter, 426 p.
https://doi.org/10.1515/9783110260373
Bader, M, ed. (2012). Kinins. Berlin: Walter de Gruyter, 386.
Sharma, J. N. (2014). Recent Developments in the Regulation of Kinins. Kuwait: Springer, 198.
https://doi.org/10.1007/978-3-319-06683-7
Tret'jakevich, Z. N., Levchin, A. M., Ancupova, V. V., & Fedota, A. M. (2013). Kallikrein-kininovaja sistema kak adaptacionno-zashhitnoe zveno organizma [Kallikrein-kinin system as an adaptation-protective link in the body]. 2013. «Sovremennaja medicina»: aktual'nye voprosy»: materialy HHІІ mezhdunarodnoj zaochnoj nauchno-prakticheskoj konferencii. Novosibirsk: SibAK, 49-58 (in Russian).
Bondarenko, Yu. I.. Prydruha, S. M., & Borys, R. M. (2013). Dynamika pokaznykiv tsytolizu ta endohennoyi intoksykatsiyi v period piznikh proyaviv travmatychnoyi khvoroby ta yikh korektsiya tiotryazolinom [Dynamics of indicators of cytolysis and endogenous intoxication in the period of late manifestations of traumatic illness and their correction by thiotriazolinum.]. Klinichna ta eksperymental'na patolohiya, 12 (1), 42-45 (in Ukrainian).
Kozak, D. V. (2012). Osoblyvosti pokaznykiv peroksydnoho okysnennia lipidiv v dynamitsi rannoho і piznoho periodu politravmy [Features of indicators of peroxide oxidation of lipids in the dynamics of the early and late period of polytrauma]. Aktualni problemy transportnoi medytsyny, 3, 103-106 (in Ukrainian).
Stupnytskyi, M. A., Zhukov, V. I., Horbach, T. V., Pavlenko, A. Yu., & Biletskyi, О. V. (2014). Dynamika kontsentratsii molekul serednoi masy v patsiientiv iz tiazhkoiu poiednanoiu torakalnoiu travmoiu v hostromu periodi travmatychnoi khvoroby [Dynamics of the concentration of medium weight molecules in patients with severe combined thoracic trauma in the acute period of traumatic illness]. Travma, 15 (4), 46-50 (in Ukrainian).
Shano, V. P., & Kucher E. A. (2011). Sindrom jendogennoj intoksikacii [Syndrome of endogenous intoxication]. Ostrye i neotlozhnye sostojanija v praktike vracha, 1, 35-41 (in Russian).
Sekela, T. Ya., Hudyma, A.A. (2007). Method for modeling polytrauma. Patent of Ukraine for useful model. MPK 2006 G 09B 23/00. № U 2007 10471; declared 21.09.2007; published 11.02.08, № 3.
Veremeenko, K.N. (1994). Proteoliticheskie fermenty i ih ingibitory. Novye oblasti primenenija v klinike [Proteolytic enzymes and their inhibitors. New areas of application in the clinic]. Vrachebnoe delo, 31, 8-13 (in Russian).
Andreichyn, M. A., Bekh, M. D., Dem'ianenko, V. V., Nychyk, A. Z., & Nychyk, N. A. (1998). Metody doslidzhennia endohennoi intoksykatsii orhanizmu: metodychni rekomendatsii [Methods of study of endogenous intoxication of an organism: Methodical recommendations]. Kyiv: MOZ Ukrainy, 10-13 (in Ukrainian).
Togajbaev, A.A., Kurguzkin, A.V., Rikun, I.V., & Karibzhanova, R.M. (1988). Sposob diagnostiki jendogennoj intoksikacii [The method of diagnosis of endogenous intoxication]. Laboratornoe delo, 9, 22-24. (in Russian).
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