Antioxidant protection and glycoproteins composition of the gastric mucosa in the experimental pathology of the gastroduodenal zone with the use of L-arginine-L-glutamate
DOI:
https://doi.org/10.31548/10.31548/dopovidi2018.04.003Abstract
The prevalence and frequency of complications in the acid-dependent diseases group (gastroesophageal reflux disease, peptic ulcer disease) prompts researchers to further study the pathophysiological factors of these diseases and their correction.The purpose of the work is to investigate the state of antioxidant protection systems and muccinum carbohydrate components composition in rats with erosive-ulcer pathology of the gastroduodenal zone under the action of L-arginine-L-glutamate. The studies were carried out on white, non-breeding male rats weighing 220-250 g. The animals were divided into three groups. I - control group (n = 6) were rats injected intragastheally through a probe physiological solution. In group ІІ (n = 6) rats with erosive-ulcerous lesions (EUL) of the gastroduodenal zone (GDZ) entered. EUL modeling was carried out according to the following scheme: for 7 days, rats received injections of medical bile (1 ml / 100 g), together with immobilization and cold stresses for 1 hour at + 4 ° C, the following 7 days weren’t subjected to stressors and were kept in conditions of vivarium with a standard diet, with withdrawal from the experiment for the 14th day. For the 7th rats of the third group (n = 6), for 7 days EUL was modeled according to the scheme described, the following 7 days of the animals received intra-abdominal injections of L-arginine-L-glutamate (20 mg / 100 g), with an exclusion from the experiment in 14 days. At the conditions of stopping influence of exogenous ulcerogenic factors leading to the formation of the erosive-ulcerative pathology of the gastroduodenal zone, after one week in the tissue of the stomach, liver and blood of experimental rats intensification of lipoperoxidation processes with depletion of the glutathione system while simultaneously changing the composition of glycoproteins with increasing sialomuccinum were found. The use of L-arginine-L-glutamate resulted in the normalization of free radical processes, the level of reduced glutathione and the quantitative and qualitative composition of glycoproteins of the stomach. A possible mechanism that affects the muccinum qualitative composition is likely to be the interaction between the oxidative-reducing cell metabolites and the transcription factors regulating expression of the MUC genes. Further research should be directed on studying the molecular mechanisms of interaction between paths of redox systems and factors influencing the proliferation and differentiation of mucosal cells of the gastroduodenal zone.
Key words: acid-dependent pathology, antioxidant defense, mucins, L-arginine-L-glutamate
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