Biological properties of risospheric bacteria pseudomonas putida and their biologically active metabolites in regulation of plant microbial systems

Abstract

Purpose. Study of the cultural-morphological and physiological and biochemical properties of Pseudomonas putida collection strains and their possible use in biotechnological developments and plant protection technologies. Methods. Biotechnological methods were used (obtaining and subcultivation of strains P. putida 8617, 8618, 8619, 8672 in vitro), microbiological (obtaining an isolated colony of bacteria, studying the growth of microbes on cups, studying the nature of growth on beveled agar, morphological, cultural, biochemical and physiological properties of experimental test cultures), statistical methods, light microscopy.. Results. The results we obtained were completely consistent with the data presented in the literature on the absorption of carbon and nitrogen, as the only source of power in the taxonomy of experimental crops. We have described that for the species differentiation Pseudomonas putida can be used and negative indicators than the similarity was demonstrated in this experiment. A very interesting feature is that the representatives of this species do not hydrolyze gelatin, it distinguishes them from other representatives of the fluorescent group of pseudodonoses. Also, the presence of a positive oxidation reaction is atypical.

The isolates which we are studying for morphological, physiological and biochemical characteristics, we attributed to the genus Pseudomonas. Scientists use carbon sources of glucose, galactose, arabinose, xylose, maltose, rhamnose, as well as reference strains as sources of carbon energy. Do not use trehalose and sucrose. At the same time, many indicators were different from those of P.syringae, a pathogen. This again demonstrates the non-affiliation of Pseudomonas putida to phytopathogens.Consequently, we determined that the collection strains Pseudomonas putida, which for a long time were stored in the collection of the Institute of Microbiology and Virology of  D.K. Zabolotniy kept their basic biological properties and can be used for further microbiological and biotechnological research.

Pseudomonas putida is saprophytic microorganisms and are potential antagonists for pathogenic bacteria and micromycetes of plants that capture the rhizosphere environment. Phytopathogenic bacteria that cause diseases of agricultural and ornamental plants are widespread in Ukraine and cause significant damage to the economy of Ukraine. Therefore, we determined the possible antagonistic effects of Pseudomonas putida on a wide range of representatives of the most common and highly aggressive phytopathogenic bacteria. The conducted experiments showed that all the experimental strains Pseudomonas putida were homogeneous, not contaminated and not mutated, retained their cultural-morphological and physiological and biochemical properties. It was determined that the collection strains Pseudomonas putida, which for a long time remained in the collection of IVB (Institute of Microbiology and Virology named after DK Zabolotny), have retained their basic biological properties and can be used for further microbiological and biotechnological research. Conclusions. According to the results of research and analysis of literary sources, Pseudomonas putida has been shown to differ from other typical representatives of its kind. The obtained results are completely consistent with the data presented in the literature on the absorption of carbon and nitrogen, as the only source of power in the taxonomy of experimental crops.