Loans of antibiotics in certificate of lips for different times of its storage and methods of processing stones

Authors

  • K. S. Myagka State Research Institute for Laboratory Diagnostics and Veterinary and Sanitary Expertise , Державний науково-дослідний інститут з лабораторної діагностики та ветеринарно-санітарної експертизи
  • S. A. Tkachuk National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/dopovidi2019.01.024

Keywords:

honey from linden, antibiotics, immunoassay analysis, storage, methods of processing beehives

Abstract

The article defines, using an ELISA enzyme immunoassay, chloramphenicol, florompenicol, neomycin and nitrofuran (AOP) in lime honey after 10 days, 30 and 120 days storage at a temperature of 25 0C in a dark place.

According to the results of the study, it was found that the residues of the studied antibiotics were within the limits established by the State monitoring plan for chloramphenicol, nitrofuran (AOP), florfenicol and neomycin.

Under the aerosol treatment of bees, a significantly lower content of the antibiotics studied than the syrup was administered. Under the aerosol treatment of bees, a significantly lower content of the antibiotics studied than the syrup was administered. Thus, after 10 days storage of honey for feeding syrup with chloramphenicol, florfenicol, neomycin and nitrofuran (AOP), their content was probably (p≤0,001) higher by 124 %, 49,5 %, 57,5 % and 77,7 % than for aerosol treatment of hives, respectively; after 30 days storage of honey for feeding syrup with chloramphenicol, florfenicol, neomycin, their content was probably (p≤0,001) higher by 125 %, 46,7 %, 63,04 %, and nitrofuran (AOP) – probably higher (p≤0,01) by 66,4 % than for aerosol treatment of hives, respectively; after 120 days storage of honey for feeding syrup with chloramphenicol, neomycin, its content was probably (p≤0,001) higher by 80,2 % and 52,2 %, while florfenicol and nitrofuran (AOP) were significantly higher (p≤0,01) by 31,7 % and 79,4 %, than for aerosol treatment of hives.

References

Galarini, R., Saluti, G., Giusepponi, D. [et all] (2015). Multiclass determination of 27 antibiotics in honey. Food Control, 48, 12–24. https://doi.org/10.1016/j.foodcont.2014.03.048

Portal RASFF. Online searchable database. URL: http://ec.europa.eu/food/food/rapidalert/rasff_portal_ database_en.htm

Holovko, A. M., Pinchuk, N. H., Dmytryieva, H. V., Kyselova, T. F. (2014). Monitorynh zalyshkiv protymikrobnykh preparativ u produktsii tvarynnytstva riznykh vyrobnykiv ta osnovni prychyny zabrudnennia [Monitoring of residues of antimicrobial agents in livestock products of different producers and the main causes of pollution Veterinary Medicine of Ukraine]. Veterynarna medytsyna Ukrainy, 8 5(219), 28–30.

Myagka, K. S., Tkachuk, S. A. (2018). State monitoring of veterinary medicinal preparations and pollutants in honey. Ukrainian Journal of Ecology, 8(2), 160–165.

Al-Waili, N, Salom, K, Al-Ghamdi, A, Ansari, M. J. (2012). Antibiotic, pesticide, and microbial contaminants of honey: human health hazards. ScientificWorld Journal, 2012, 930–849 [Pub Med]. https://doi.org/10.1100/2012/930849

Čuláková, V, Kiss, E, Kubincová, J, Šilhár, S. (2008). Kinetics of degradation of oxytetracycline and tetracycline in honey and in a glucose-fructose model mixture in various storage condition. Journal Food Nutrition, 47, 139–43.

Peres, G. T, Rath, S, Reyes, F. G. R. (2010). A HPLC with fluorescence detection method for the determination of tetracyclines residues and evaluation of their stability in honey. Food Control, 21, 620–5. https://doi.org/10.1016/j.foodcont.2009.09.006

Dumitrel, G.-A., Glevitzky, M., Perju, D., Cara, M. C. (2012). Stability of tetracycline residues in honey Journal Serbian Chemistry, 77 (7), 879–886. https://doi.org/10.2298/JSC111002214C

Scortichini, G., Annunziata, L., Haouet, M. N., Benedetti, F., Krusteva, I., Galarini, R. (2005). ELISA qualitative screening of chloramphenicol in muscle, eggs, honey and milk: method validation according to the Commission Decision 2002/657/EC criteria. Analytica Chimica Acta, 535, 1–2, 43–48. https://doi.org/10.1016/j.aca.2004.12.004

Tao, X., Jiang, H., Zhu, J., Wang, X., Wang, Z., Niu, L. (2014). An ultrasensitive chemiluminescent ELISA for determination of chloramphenicol in milk, milk powder, honey, eggs and chicken muscle. Journal Food and Agricultural Immunology, 25, 1, 137–148. https://doi.org/10.1080/09540105.2012.753513

Lopez, M. I., Feldlaufer, M. F., Williams, A. D., Chu, P.-S. (2007). Determination Confirmation of Nitrofuran Residues in Honey Using LC-MS/MS. Journal Agricultural Food Chemistry, 55(4), 1103–1108. https://doi.org/10.1021/jf0625712

O’Mahony, J., Moloney, M. McConnell, R. I., Benchikh, El O., Lowry, P., Furey, A., Danaher, M. (2011). Simultaneous detection of four nitrofuran metabolites in honey using a multiplexing biochip screening assay. Biosensors and Bioelectronics, 26, 10, 4076–4081. https://doi.org/10.1016/j.bios.2011.03.036

CARA M.C. [et all]. (2013). Degradation of Streptomycin in Honey. Food Technology and Biotechnology, 51 (3), 429–433

Moreno-González, D., Lara, F. J., Jurgovská, N., Gámiz-Gracia, L., García-Campaña, A. M. (2015). Determination of aminoglycosides in honey by capillary electrophoresis tandem mass spectrometry and extraction with molecularly imprinted polymer. Analytica Chimica Acta, 891, 321–328. https://doi.org/10.1016/j.aca.2015.08.003

Published

2019-02-28

Issue

Section

Veterinary medicine, quality and safety of livestock products