Influence of membrane-repairing medications on the expression of proteins of plasmolemma of enterocytes during the formation of colostral immunity

Authors

  • S.I. Golopura National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • Tsvilikhovsky M.I. National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • Popadiuk B.V. National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/dopovidi2019.06.019

Keywords:

newborn calves, small intestine, enterocyte, plasmolemma, proteins, phospholipids, native liposomes, colostral immunity

Abstract

The indices of the content of proteins with molecular mass of 10 – 15 and 15 – 24 kDa in the plasmolemma of enterocytes of the small intestine of newborn calves in the dynamics  from the birth till the age of 1 day were investigated. The study was performed on three groups of calves (the control group and two experimental ones) of Ukrainian black-and-white dairy breed by drawing the samples of enterocytes of jejunum and determining protein content of membrane lysate fraction by electrophoresis. The application of native liposomes based on soybean lecithin and “Membranostabil” medication for newborn calves with colostrum increases the expression of plasmolemic proteins of enterocytes with a molecular mass of 10 – 15 kDa. The dynamics of comparative analysis between fractions of protein expression with molecular mass of 10 – 15 and 15 – 24 kDa in the plasmolemma of the enterocytes of the jejunum of control and experimental groups of calves is shown. The result of application of native liposomes with phospholipid bilayer based on soybean lecithin and “Membranostabil” medication allows to assume the positive effect of these means on the level of colostral immunoglobulins in the serum of newborn calves.

References

Tsvilikhovskyi, M.I., Maryniuk, M.O., Golopura S.I., Avdieieva, L.Ju., Nemova, T.V., Yakymchuk, O.M., Zhukotskyi, E.K. (2014) Veterinary drug "Membranostabil" Patent of Ukraine for useful model. MPK A61K9/127. № u201402508; declared. 13.03.2014; published 10.09.2014, № 17.

Schägger H., von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry. 1987, vol. 166, no. 2. PP. 368-379.

https://doi.org/10.1016/0003-2697(87)90587-2

Marynyuk M.O., Holopura S. I., Yakymchuk O. M., Nemova T. V., Tsvilihovsky M. I. Level of colostral immunity and development of digestive disorders in newborn calves.Veterinarna Medicina Ukrainy, 2014, no 5, pp. 21-23. (in Ukrainian)

Tereshchenko M. P. Age peculiarities of the protein composition of the plasma membrane of the small intestine epithelium in cattle // Dis. biol. N. 03.00.04. - biochemistry. Kyiv, NAU. - 1996. - 162 p.

Golopura S. I. Tsvilihovsky M. I. The correction of total content of protein and urea in the serum of blood of newborn calves in a period formation of colostral immunity.Visnyk Poltavskoy Derzh. Academy, 2014, no 3, pp. 95-97. (in Ukrainian)

Singh, A. K., Pandita, S., Vaidya, M. M., Chandra, G. & Kushwaha, R. Colostral immunoglobulins and neonatal immunity in bovine // Wayamba Journal of Animal Science. ISSN: 2012-578X; P78-P84 2011.

Holloway, N. M., Tyler, J. W., Lakritz, J., Carlson, S. L. and Holle, J. 2001. Serum immunoglobulinG concentrations in calves fed fresh and frozen colostrum. J. Am. Vet. Med. Assoc. 219: 357-359. Huang, Y., Shao, X. M., Neu, J. 2003. Immunonutrients and neonates. Eur. J. Pediatr. 162: 122-128.

https://doi.org/10.2460/javma.2001.219.357

Larson, B. L., Heary, H. L. and Devery, J. E. Immunoglobulin production and transport by the mammary gland. J. Dairy Sci. 1980. 63: 665671.

https://doi.org/10.3168/jds.S0022-0302(80)82988-2

Egungula N.K., Egurg U.S., Managan R.C. et al. Intestinal brush boder calmodulin: key role in the regulation of NaCl transport in Giardia Lamblia infected mice // Biochen. int. 1987. Vol. 14, N2. -P. 249-256.

William Strohl Lila Strohl. Therapeutic Antibody Engineering // eBook, Woodhead Publishing. 2012. 696 p

https://doi.org/10.1533/9781908818096

Downloads

Published

2019-12-28

Issue

No. 6(82) (2019)

Section

Veterinary medicine, quality and safety of livestock products

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).