Microscopic structure of pig liver for use in feeding of natural feed additives LG-MAX and Sel-Plex
DOI:
https://doi.org/10.31548/dopovidi2021.04.012Keywords:
young pigs, liver, microscopic structure, feed additives LG-MAX and Sel-PlexAbstract
The use of feed additives consisting of organic components and containing fatty acids, namely polyunsaturated fatty acids, of the omega-3 family, is necessary for the regulation of numerous biological processes and support of the body's metabolic homeostasis.
The material for this study was young meat-lard pigs (Landrace + Large White) and muscle tissue samples from the longissimus dorsi (m. Longisimus dorsi) pigs taken at the level of 10-12 thoracic vertebrae, at the time of slaughter, at the end of the study period.
For histological examination, the selected material was fixed in a 10% formalin solution according to Lilly, embedded in paraffin, and histosections with a thickness of 10 μm were made, which were stained with Karatsi's hematoxylin and eosin. The obtained preparations were examined under a light microscope with a Micros MCQ-2000 photo attachment.
The liver of the pigs in the experimental groups did not differ in appearance from the liver of the pigs in the control group. However, according to the histological examination of the liver of the pigs of the experimental group, which were fed the feed additive LG-MAX 2.0 g / day, the presence of single dystrophic processes in hepatocytes was established in the liver of the pigs of the experimental group, which were fed the feed additive LG-MAX 4.0 g / day - the presence of granular and fatty infiltrative dystrophy and vascular hyperemia inside the lobule, and in the liver of pigs that were fed the feed additive LG-MAX 2.0 g / day together with Sel-Plex - in addition, a weakly expressed lymphohistiocytic infiltration of the stroma was established.
References
Kozlov, S. V. (2018). Novye metody farmakologicheskoj korrekcii i profilaktiki zabolevanij pecheni u sel'skohozyajstvennyh i melkih neproduktivnyh zhivotnyh [New methods of pharmacological correction and prevention of liver diseases in agricultural and small unproductive animals]: dis… d. vet. nauk: 06.02.01 / Saratov. gos. agrarn. un-t imeni N. I. Vavilova. Saratov, 357 s. [in Russia].
Vondruskova, H., Slamova, R., Trckova, M., Zraly, Z., Pavlik, I. (2010). Alternatives to antibiotic growth promoters in prevention of diarrhoea in weaned piglets: a review. Veterinarni Medicina. 55 (5), P. 199-224. https://doi.org/10.17221/2998-VETMED
Stanley, W. C., Cox, J. W., Asemu, G., O'Connell, K. A., Dabkowski, E. R., Xu, W., Ribeiro, Jr. R. F., Shekar, K. C., Hoag, S. W., Rastogi, S., Sabbah, H. N., Daneault, C., Rosiers, C. (2013). Evaluation of docosahexaenoic acid in a dog model of hypertension induced left ventricular hypertrophy. Journal of cardiovascular translational Research. 6, 1000-1010. https://doi.org/10.1007/s12265-013-9511-y
Krugera, M. C., Coetzee, M., Haag, M., Weiler, H. (2010). Long-chain polyunsaturated fatty acids: Selected mechanisms of action on bone. Progress in Lipid Research. 49, 4. 438-449. https://doi.org/10.1016/j.plipres.2010.06.002
Vallim, T., Salter, A. M. (2011). Regulation of hepatic gene expression by saturated fatty acids. Chemistry, Medicine. 82, 4-6, 211-218. https://doi.org/10.1016/j.plefa.2010.02.016
Lunney, J. K. (2007). Advances in swine biomedical model genomics. Journal of Biological Sciences. 3, 179-184. https://doi.org/10.7150/ijbs.3.179
Szostak, A., Ogłuszka, M., Pas, M. F. W., Poławska, E., Urbański, P., Juszczuk-Kubiak, E., Blicharski, T., Pareek, C. S., Dunkelberger, J. R., Horbańczuk, J. O., Pierzchała, M. (2016). Effect of a diet enriched with omega-6 and omega-3 fatty acids on the pig liver transcriptome. Genes & Nutrition. 11, 9, 1-17. https://doi.org/10.1186/s12263-016-0517-4
Nguyen, P., Leray, V., Diez, M., Serisier, S., Le Bloc'h, J., Siliart, B., Dumon, H. (2008). Liver lipid metabolism. Journal Animal Physiology and Animal Nutrition. 92, 272-283. https://doi.org/10.1111/j.1439-0396.2007.00752.x
Birta, H. O., Burhu, Yu. H. (2011). Doslidzhennia mikroskopichnoi budovy pechinky ta holovnoho mozku svynei yak pokaznyk ekolohichnoi bezpeky [Study of the microscopic structure of the liver and brain of pigs as an indicator of environmental safety]. Visnyk Poltavskoi derzhavnoi ahrarnoi akademii. 3, 60-62. [in Ukrainian].
Huralska, S. V., Horalskyi, L. P., Horalska, I. Yu. (2014). Mikroskopichna budova ta morfometrychni pokaznyky pechinky domashnikh tvaryn [Microscopic structure and morphometric parameters of the liver of domestic animals]. Visnyk ZhNAEU. 2 (42), 1, 160-164. [in Ukrainian].
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