Research of microcystins as inhibitors of Phytophthora infestans development
DOI:
https://doi.org/10.31548/dopovidi2021.05.001Keywords:
microcystin, toxicity, inhibitors, phytophthora, in vitro, biotesting, test object, in vivo, biosafetyAbstract
Information from foreign literary sources regarding microcystins of blue-green algae is presented. The current state of the problem of reservoirs «blooming» and the significance of this phenomenon for humans are reflected. The research was carried out in two stages: laboratory and full-scale. A pure culture of Phytophthora infestans was isolated, on which further studies were carried out. Isolation of isolates was carried out on agar nutrient medium. Potassium permanganate and ethanol were selected from the available antiseptics. Field experiments were carried out on experimental lines of Solanum lycopersicum by diagnosing signs of late blight disease. The determination of the potential negative effect of tomato plants treated with a suspension of cyanobacteria was carried out by the method of biotesting using Achatina fulica as a test object. The effect of blue-green algae toxins – microcystins – on colonies Ph. infestans in vitro is described. Photometric observation of the decrease in the number of colonies with a time interval of three days is presented. The phytophtorostatic effect of microcystins has been established. Degradation of Ph. infestans the next day were fixed after treatment of the colony with a suspension of cyanobacteria. An inhibitory effect was revealed, almost to the complete disappearance of symptoms in plants partially affected by late blight and grown in vivo. It has been determined that plants treated with microcystin can be considered safe for further consumption; the death of model organisms has not been registered.
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