Prooxidant-antioxidant homeostasis in the incubed sperm of boars at feeding lactates of microelements

Authors

DOI:

https://doi.org/10.31548/dopovidi2020.02.017

Keywords:

sperm, boars, incubation, peroxide oxidation, microelements, vitamins, antioxidants

Abstract

Microelements - limiting micronutrients, being closely linked to enzymes, vitamins and hormones, cause metabolic transformations and provide the formation of reproductive function in pigs.

The purpose of the research was to determine the peculiarities of the formation of prooxidant-antioxidant homeostasis in incubated sperm of boars during feeding of micro element lactates.

In the study it was used adult boars of the Large White breed. The duration of the experiment was 120 days, including: preparatory - 30 days, basic - 60 (feeding of zinc, magnesium, selenium, copper and iron) and final - 30 days. In the main period of the experiment, the diet of the animals in the control group remained unchanged, and the two experimental ones was with the addition of zinc, magnesium, selenium, copper and iron. The level of biologically active components in the diet of the experimental groups was higher by 10% and 20% compared with the control group. The obtained ejaculate samples were incubated at temperatures of + 38°C, + 17°C and + 5°C for three hours.

It was found out the fact that the supplemental feeding of zinc, magnesium, selenium, copper and iron lactates in the feed mixture to boars  significantly altered the prooxidant-antioxidant homeostasis in ejaculates at different storage temperatures. The incubation of sperm samples at physiologically normal temperature is accompanied by a significant acceleration of peroxidation processes, and when reduced to + 5°C their inhibition occurs.

The formation of prooxidant-antioxidant homeostasis in sperm is significantly correlated with the number of microelements of lactates additionally fed. The addition of these biologically active substances on 10% above normal after 60 days of feeding helps to preserve the content of vitamins of antioxidant action, restored glutathione, stimulates the activity of superoxide dismutase and catalase and is accompanied by the slowing of peroxidation processes.

Adding microelements lactates to the feed mix is on 20% more than the norma for boars in comparison after 30 days of use, stimulates peroxidation processes, intensifies the use of non-enzymatic and activates enzyme antioxidants.

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Published

2020-04-20

Issue

No. 2(84) (2020)

Section

Technology of production and processing of livestock products

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